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Titre du document / Document title

Using pulse field gradient NMR diffusion measurements to define molecular size distributions in glycan preparations

Auteur(s) / Author(s)

MILLER Michelle C. ; KLYOSOV Anatole ; PLATT David ; MAYO Kevin H. ;

Résumé / Abstract

Glycans comprise perhaps the largest biomass in nature, and more and more glycans are used in a number of applications, including those as pharmaceutical agents in the clinic. However, defining glycan molecular weight distributions during and after their preparation is not always straightforward. Here, we use pulse field gradient (PFG) 1H NMR self-diffusion measurements to assess molecular weight distributions in various glycan preparations. Initially, we derived diffusion coefficients, D, on a series of dextrans with reported weight-average molecular weights from about 5 kDa to 150 kDa. For each dextran sample, we analyzed 15 diffusion decay curves, one from each of the 15 major 1H resonance envelopes, to provide diffusion coefficients. By measuring D as a function of dextran concentration, we determined D at infinite dilution, Dinf, which allowed estimation of the hydrodynamic radius, Rh, using the Stokes-Einstein relationship. A plot of log Dinf versus log Rh was linear and provided a standard calibration curve from which Rh is estimated for other glycans. We then applied this methodology to investigate two other glycans, an α-(1→2)-L-rhamnosyl-α-(1→4)-D-galacturonosyl with quasi-randomly distributed, mostly terminal β(1→4)-linked galactose side-chains (GRG) and an α(1→6)-D-galacto-β(1→4)-D-mannan (Davanat), which is presently being tested against cancer in the clinic. Using the dextran-derived calibration curve, we find that average Rh values for GRG and Davanat are 76 ± 6 x 10―10 m and 56 ± 3 x 10―10 m, with GRG being more polydispersed than Davanat. Results from this study will be useful to investigators requiring knowledge of polysaccharide dispersity, needing to study polysaccharides under various solution conditions, or wanting to follow degradation of polysaccharides during production.

Revue / Journal Title

Carbohydrate research    ISSN  0008-6215   CODEN CRBRAT 

Source / Source

2009, vol. 344, no10, pp. 1205-1212 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Oxford, ROYAUME-UNI  (1965) (Revue)

Mots-clés d'auteur / Author Keywords

NMR

;

Diffusion

;

Carbohydrate

;

Glycan

;

Localisation / Location

INIST-CNRS, Cote INIST : 12339, 35400018725839.0080

Nº notice refdoc (ud4) : 21696082



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