Titre du document / Document title
PRODUCTION OF PHENOLIC COMPOUNDS BY Spirulina maxima MICROALGAE AND THEIR PROTECTIVE EFFECTS IN VITRO TOWARD HEPATOTOXICITY MODEL
Auteur(s) / Author(s)ABD EL-BAKY Hanaa H.
EL BAZ Farouk K.
EL-BAROTY Gamal S.
Résumé / Abstract
The purpose of this study was to illustrate the enhancing process of phenolics' synthesis in Spirulina maxima grown in Zarrouk's medium supplemented with different concentrations of NaNO3
and/or combined with phenylalanine (L-PA). Also, the protective efficacy of Spirulina polyphenolic (SPP) extracts against CCI4
-induced microsomal lipid peroxidation and scavenging of hydroxyl radical formation were performed. The results revealed that the levels of NaNO3
and L-PA in growth medium have positive effects on the production of biomass (34-64 mgday-1
), total phenolics (4.51-16.96 mgg-1
d.w) and flavonoids (1.32-5.12 mgg-1
d.w). The highest levels of these compounds were obtained in Zarrouk's medium containing 3.77 gL-1
and 100 mgL-1
L-PA. The HPLC-DAD profile of all phenolic extracts of Spirulina showed the presence of a large number of phenolic acids and flavonoids, in variable levels. Gallic, chlorogenic, cinnamic, pinostrobin and p-OH-benzoic acids were found as the most abundant constituents among different extracts. Polyphenolic compounds of Spirulina exhibited antioxidant effects on CCI4
-induced lipid peroxidation (inhibition %) in liver homogenate and on DPPH radical scavenging activity (with IC50
values ranging between 23.22 - 35.62 μgml-1
), in dose-dependent manner. Their protective potential was comparable to that of standard phenolic antioxidants (BHT, BHA and α-tocopherol, with IC50
values of 13.22 - 23.62 μgml-1
). Thus, Spirulina maxima can be regarded as potent natural source of nutraceutical and functional ingredients.
Revue / Journal TitleAdvances in food sciences
Source / Source
2009, vol. 31, no
1, pp. 8-16 [9 page(s) (article)]
Langue / Language
Revue : Allemand
Editeur / Publisher
Parlar Scientific Publications, Freising, ALLEMAGNE
Mots-clés d'auteur / Author Keywords
Localisation / Location
INIST-CNRS, Cote INIST : 15792, 35400018847898.0020
Nº notice refdoc (ud4) : 21511068