Titre du document / Document title

PRODUCTION OF PHENOLIC COMPOUNDS BY Spirulina maxima MICROALGAE AND THEIR PROTECTIVE EFFECTS IN VITRO TOWARD HEPATOTOXICITY MODEL

Auteur(s) / Author(s)

ABD EL-BAKY Hanaa H. ; EL BAZ Farouk K. ; EL-BAROTY Gamal S. ;

Résumé / Abstract

The purpose of this study was to illustrate the enhancing process of phenolics' synthesis in Spirulina maxima grown in Zarrouk's medium supplemented with different concentrations of NaNO₃ and/or combined with phenylalanine (L-PA). Also, the protective efficacy of Spirulina polyphenolic (SPP) extracts against CCI₄-induced microsomal lipid peroxidation and scavenging of hydroxyl radical formation were performed. The results revealed that the levels of NaNO₃ and L-PA in growth medium have positive effects on the production of biomass (34-64 mgday^-1), total phenolics (4.51-16.96 mgg^-1 d.w) and flavonoids (1.32-5.12 mgg^-1 d.w). The highest levels of these compounds were obtained in Zarrouk's medium containing 3.77 gL^-1 NaNO₃ and 100 mgL^-1 L-PA. The HPLC-DAD profile of all phenolic extracts of Spirulina showed the presence of a large number of phenolic acids and flavonoids, in variable levels. Gallic, chlorogenic, cinnamic, pinostrobin and p-OH-benzoic acids were found as the most abundant constituents among different extracts. Polyphenolic compounds of Spirulina exhibited antioxidant effects on CCI₄-induced lipid peroxidation (inhibition %) in liver homogenate and on DPPH radical scavenging activity (with IC₅₀ values ranging between 23.22 - 35.62 μgml^-1), in dose-dependent manner. Their protective potential was comparable to that of standard phenolic antioxidants (BHT, BHA and α-tocopherol, with IC₅₀ values of 13.22 - 23.62 μgml^-1). Thus, Spirulina maxima can be regarded as potent natural source of nutraceutical and functional ingredients.

Revue / Journal Title

Advances in food sciences    ISSN  1431-7737 

Source / Source

2009, vol. 31, n^o1, pp. 8-16 [9 page(s) (article)]

Langue / Language

Anglais
Revue : Allemand

Editeur / Publisher

Parlar Scientific Publications, Freising, ALLEMAGNE  (1996) (Revue)

Mots-clés d'auteur / Author Keywords

Spirulina maxima

;

antioxidant activity

;

carbon tetrachloride

;

hepatoprotective effects

;

Localisation / Location

INIST-CNRS, Cote INIST : 15792, 35400018847898.0020