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Titre du document / Document title

Ultrastructural changes to the tegumental system and the gastrodermal cells in adult Fasciola hepatica following in vivo treatment with the experimental fasciolicide, compound alpha

Auteur(s) / Author(s)

MCCONVILLE M. (1) ; BRENNAN G. P. (1) ; FLANAGAN A. (1) ; EDGAR H. W. J. (2) ; CASTILLO R. (3) ; HERNANDEZ-CAMPOS A. (3) ; FAIRWEATHER I. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Parasite Proteomics and Therapeutics Research Group, Queens UniversityBelfast, ETATS-UNIS
(2) Veterinary Sciences Division, Agri-Food Biosciences Institute (AFBI), Stormont, Belfast, ETATS-UNIS
(3) Facultads de Quimica y Medicina Veterinaria, Universidad Nacional Autonoma de Mexico (UNAM), Mexico City, MEXIQUE

Résumé / Abstract

Sheep infected with the triclabendazole-susceptible, Cullompton isolate of Fasciola hepatica were dosed with 15 mg/kg of compound alpha at 12 weeks post-infection. Adult flukes were recovered from the bile ducts at 24, 48 and 72 h posttreatment (p.t.). Ultrastructural changes to the flukes were assessed using transmission electron microscopy (TEM), with a view to gathering information on the mechanism(s) of action for compound alpha and on the possible route of its entry into F. hepatica. The tegumental syncytium was more severely affected than the gut at all time-points p.t. with compound alpha, suggesting a predominantly trans-tegumental route of uptake. Disruption to the tegumental system became increasingly severe over time. A stress response was observed at 24 h p.t. and took the form of blebbing and increases in the production and transport of secretory bodies. By 72 h p.t., extensive tegumentalloss and degeneration of the tegumental cell bodies had occurred. Degeneration of subtegumental tissues and internal flooding were also observed. Changes in the gastrodermal cells were slow to develop: reduced secretory activity was evident at 72 h p.t.. There was progressive disruption to the somatic muscle layers, with disorganization of the muscle blocks and loss of muscle fibres.

Revue / Journal Title

Parasitology    ISSN  0031-1820   CODEN PARAAE 

Source / Source

2009, vol. 136 (6), pp. 665-680 [16 page(s) (article)] (1 p.3/4)

Langue / Language

Anglais

Editeur / Publisher

Cambridge University Press, Cambridge, ROYAUME-UNI  (1908) (Revue)

Mots-clés anglais / English Keywords

Invertebrata

;

Helmintha

;

Plathelmintha

;

Trematoda

;

Fasciola hepatica

;

Parasite

;

Transmission electron microscopy

;

Liver

;

Experimental study

;

In vivo

;

Mots-clés français / French Keywords

Invertebrata

;

Helmintha

;

Plathelmintha

;

Trematoda

;

Fasciola hepatica

;

Parasite

;

Microscopie électronique transmission

;

Foie

;

Etude expérimentale

;

In vivo

;

Mots-clés espagnols / Spanish Keywords

Invertebrata

;

Helmintha

;

Plathelmintha

;

Trematoda

;

Fasciola hepatica

;

Parásito

;

Microscopía electrónica transmisión

;

Hígado

;

Estudio experimental

;

In vivo

;

Mots-clés d'auteur / Author Keywords

Fasciola hepatica

;

liver fluke

;

compound alpha

;

transmission electron microscopy

;

Localisation / Location

INIST-CNRS, Cote INIST : 3187, 35400018498627.0090

Nº notice refdoc (ud4) : 21416665



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