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Titre du document / Document title

Identification of a novel chimeric gene, orf725, and its use in development of a molecular marker for distinguishing among three cytoplasm types in onion (Allium cepa L.)

Auteur(s) / Author(s)

KIM Sunggil (1) ; LEE Eul-Tai (2) ; DONG YOUN CHO (3) ; HAN Taeho (1) ; BANG Haejeen (4) ; PATIL Bhimanagouda S. (4) ; YUL KYUN AHN (5) ; YOON Moo-Kyoung (5) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University, Gwangju 500-757, COREE, REPUBLIQUE DE
(2) Mokpo Experiment Station, National Institute of Crop Science, RDA, Muan 534-833, COREE, REPUBLIQUE DE
(3) ONBREETECH Corp, Haenam 536-803, COREE, REPUBLIQUE DE
(4) Department of Horticultural Sciences, Vegetable and Fruit Improvement Center, Texas A&M University, College Station, TX 77845, ETATS-UNIS
(5) National Horticultural Research Institute, RDA, Suwon 441-440, COREE, REPUBLIQUE DE

Résumé / Abstract

A novel chimeric gene with a 5' end containing the nearly complete sequence of the coxl gene and a 3' end showing homology with chive orfA501 was isolated by genome walking from two cytoplasm types: CMS-S and CMS-T, both of which induce male-sterility in onion (Allium cepa L.). In addition, the normal active and variant inactive coxl genes were also isolated from onions containing the normal and CMS-S cytoplasms, respectively. The chimeric gene, designated as orf725, was nearly undetectable in normal cytoplasm, and the copy number of the normal coxl gene was significantly reduced in CMS-S cytoplasm. RT-PCR results showed that orf725 was not transcribed in normal cytoplasm. Meanwhile, the normal coxl gene, which is essential for normal mitochondrial function, was not expressed in CMS-S cytoplasm. However, both orf725 and coxl were transcribed in CMS-T cytoplasm. The expression of orf725, a putative male-sterility-inducing gene, was not affected by the presence of nuclear restorer-of-fertility gene(s) in male-fertility segregating populations originating from the cross between a male-sterile plant containing either CMS-T or CMS-S and a male-fertile plant whose genotypes of nuclear restorer gene(s) might be heterozygous. The specific stoichiometry of orf725 and coxl in the mtDNA of the three cytoplasm types was consistent among diverse germplasm. Therefore, a molecular marker based on the relative copy numbers of orf725 and coxl was designed for distinguishing among the three cytoplasm types by one simple PCR. The reliability and applicability of the molecular marker was shown by testing diverse onion germplasm.

Revue / Journal Title

Theoretical and applied genetics    ISSN  0040-5752   CODEN THAGA6 

Source / Source

2009, vol. 118, no3, pp. 433-441 [9 page(s) (article)] (3/4 p.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1968) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Liliaceae

;

Agriculture

;

Genetics

;

Allium cepa

;

Cytoplasm

;

Molecular marker

;

Development

;

Use

;

Gene

;

Identification

;

Mots-clés français / French Keywords

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Liliaceae

;

Agriculture

;

Génétique

;

Allium cepa

;

Cytoplasme

;

Marqueur moléculaire

;

Développement

;

Utilisation

;

Gène

;

Identification

;

Mots-clés espagnols / Spanish Keywords

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Liliaceae

;

Agricultura

;

Genética

;

Allium cepa

;

Citoplasma

;

Marcador molecular

;

Desarrollo

;

Uso

;

Gen

;

Identificación

;

Localisation / Location

INIST-CNRS, Cote INIST : 395, 35400019612481.0040

Nº notice refdoc (ud4) : 21095744



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