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Titre du document / Document title

l-Methioninase Production by Filamentous Fungi: I-Screening and Optimization Under Submerged Conditions

Auteur(s) / Author(s)

Khalaf Salwa A. (1) ; El-Sayed Ashraf S. A. (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt
(2) Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Kyoto, Sakyo-ku, Japan

Résumé / Abstract

Findings show 21 fungal isolates belonging to eight genera recovered from Egyptian soils that have the potential to attack l-methionine under submerged conditions. Aspergillus flavipes had the most methioninolytic activity, giving the highest yield of l-methioninase (10.78 U/mg protein), rate of methionine uptake (93.0%), and growth rate (5.0 g/l), followed by Scopulariopsis brevicaulis and A. carneus. The maximum l-methioninase productivity (11.60 U/mg protein) by A. flavipes was observed using l-methionine (0.8%) as an enzyme-inductive agent and glucose (1%) as a co-dissimilated carbon source. A significant reduction in l-methioninase biosynthesis by A. flavipes was detected using carbon-free medium, suggesting the lack of ability to use l-methionine as a carbon and nitrogen source. Potassium dihydrogen phosphate (0.25%), the best source of phosphorus, favors enzyme biosynthesis and enhances the level of methionine uptake by A. flavipes. The maximum l-methioninase productivity (12.58 U/mg protein) and substrate uptake (95.6%) were measured at an initial pH of 7.0.

Revue / Journal Title

Current microbiology    ISSN  1432-0991 

Source / Source

2009, vol. 58, no3, pp. 219-226 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1996) (Revue)

Localisation / Location

35400060142784.0001

Springer Science+Business Media, LLC, 2009
Nº notice refdoc (ud4) : 21013699



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