Titre du document / Document title
A ribosome-inactivating protein from Amaranthus viridis
Auteur(s) / Author(s)
KWON S.-Y. (1) ;
AN C. S. (2) ;
LIU J. R. (1) ;
PAEK K.-H. (3) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Korea Research Institute of Bioscience and Biotechnology, P. O. Box 115, Taejon 305-333, COREE, REPUBLIQUE DE
(2) Department of Biology, Seoul National University, Seoul 151-742, COREE, REPUBLIQUE DE
(3) Graduate School of Biotechnolagy, Korea University, Seoul 136-701, COREE, REPUBLIQUE DE
Résumé / Abstract
An antiviral protein purified from the leaves of Amaranthus viridis was named amaranthin. The in vivo antiviral activity of amaranthin was confirmed in tobacco mosaic virus infection test on Nicotiana glutinosa leaves. The molecular mass of the amaranthin was estimated about 30 kDa 4y SDS-PAGE and the pI was measured as 9.8 by isoelectric focusing (lEF) analysi-s. of the amaranthin using in vitro translation inhibition assay was similar to that of pokeweed antiviral protein (PAP) IC
50 of 25pM. Depurination activity (N-glycosidase activity) against animal rRNA was also confirmed.
Revue / Journal Title
Bioscience, biotechnology, and biochemistry
ISSN
0916-8451
Source / Source
1997, vol. 61, n
o9, pp. 1613-1614 (11 ref.)
Langue / Language
Anglais
Editeur / Publisher
Japan Society for Bioscience Biotechnology and Agrochemistry, Tokyo, JAPON
(1992)
(Revue)
Mots-clés anglais / English Keywords
Medicinal plant ;
Defense mechanism ;
Plant pathogen ;
Host virus relation ;
In vitro ;
Ribosome inactivating protein ;
Antiviral ;
Plant leaf ;
rRNA N-glycosidase ;
Pharmacognosy ;
Plant origin ;
Vegetable crop ;
In vivo ;
Virus ;
N-Glycosidases ;
Glycosidases ;
Hydrolases ;
Enzyme ;
Amaranthaceae ;
Dicotyledones ;
Angiospermae ;
Spermatophyta ;
Mots-clés français / French Keywords
Plante médicinale ;
Mécanisme défense ;
Phytopathogène ;
Relation hôte virus ;
In vitro ;
Protéine inactivant ribosome ;
Antiviral ;
Feuille végétal ;
rRNA N-glycosidase ;
Pharmacognosie ;
Origine végétale ;
Plante légumière ;
In vivo ;
Virus ;
Amaranthus viridis ;
Amaranthine ;
N-Glycosidases ;
Glycosidases ;
Hydrolases ;
Enzyme ;
Amaranthaceae ;
Dicotyledones ;
Angiospermae ;
Spermatophyta ;
Mots-clés espagnols / Spanish Keywords
Planta medicinal ;
Mecanismo defensa ;
Fitopatógeno ;
Relación huesped virus ;
In vitro ;
Proteína inactivante ribosoma ;
Antiviral ;
Hoja vegetal ;
rRNA N-glycosidase ;
Farmacognosia ;
Origen vegetal ;
Planta leguminosa ;
In vivo ;
Virus ;
N-Glycosidases ;
Glycosidases ;
Hydrolases ;
Enzima ;
Amaranthaceae ;
Dicotyledones ;
Angiospermae ;
Spermatophyta ;
Localisation / Location
INIST-CNRS, Cote INIST : 8935, 35400007959738.0440
Nº notice refdoc (ud4) : 2089335
