Titre du document / Document title

Noradrenaline-induced lipolysis in isolated mesenteric, omental and subcutaneous adipocytes from obese subjects

Auteur(s) / Author(s)

VAN HARMELEN V. ⁽¹⁾ ; LÖNNQVIST F. ⁽¹⁾ ; THÖRNE A. ⁽²⁾ ; WENNLUND A. ⁽²⁾ ; LARGE V. ⁽¹⁾ ; REYNISDOTTIR S. ⁽¹⁾ ; ARNERL P. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Medicine, Research Center, Karolinska Institute, Huddinge University Hospital, Stockholm, SUEDE
⁽²⁾ Department of Surgery, Research Center, Karolinska Institute, Huddinge University Hospital, Stockholm, SUEDE

Résumé / Abstract

OBJECTIVE: The action of noradrenaline on human mesenteric, omental and subcutaneous adipocytes was compared. We also determined whether regional differences in the noradrenaline-effect were linked to variations in adrenoceptor subtype function. DESIGN: The lipolytic effects of different concentrations of noradrenaline (β1-, β2-, β3- and α2-adrenoceptor agonist), isoprenaline (β1-, β2- and β3-adrenoceptor agonist) and selective β1-, β2- and β3-adrenoceptor agonists (dobutamine, terbutaline and CGP 12177, respectively) were studied in adipocytes isolated from the three adipose tissue regions in the same subject. In addition, the effect of the α2-adrenoceptor antagonist, yohimbine, was studied on noradrenaline-induced glycerol release. SUBJECTS: Thirteen otherwise healthy obese subjects (nine females, four males). RESULTS: The noradrenaline-induced lipolytic response did not differ between omental and mesenteric adipocytes but was 50% higher than in subcutaneous adipocytes (P < 0.05). Furthermore, noradrenaline sensitivity and intrinsic activity (in relation to isoprenaline) were higher in the two visceral fat cells than in the subcutaneous fat cells. The intrinsic activity of noradrenaline increased close to that of isoprenaline when yohimbine was added to the incubation system. Isoprenaline sensitivity was five times higher in the two visceral fat cells than in the subcutaneous fat cells. For CGP 12177, sensitivity and intrinsic activity did not differ between mesenteric and omental adipocytes, but was higher in these two regions when compared to subcutaneous adipocytes. For dobutamine and terbutaline no significant regional differences were found. CONCLUSION: β3-adrenoceptor action is enhanced and «2-adrenoceptor action is decreased in both mesenteric and omental adipocytes as compared to subcutaneous adipocytes. However, the two visceral fat depots show no difference in adrenoceptor function. The difference in β3- and α2-adrenoceptor function might explain why noradrenaline induced lipolysis is increased in the two visceral fat depots, as compared to the subcutaneous fat depot.

Revue / Journal Title

International journal of obesity   ISSN 0307-0565   CODEN IJOBDP 

Source / Source

1997, vol. 21, n^o11, pp. 972-979 (25 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing, Basingstoke, ROYAUME-UNI  (1977) (Revue)

Mots-clés anglais / English Keywords

Obesity ; Adipose tissue ; Norepinephrine ; Lipolysis ; Subcutaneous tissue ; Adipocyte ; Mesentery ; Omentum ; Hormonal receptor ; β-Adrenergic receptor agonist ; Comparative study ; Influence ; Human ; Nutritional status ; Nutrition disorder ; Catecholamine ; Adrenal hormone ;

Mots-clés français / French Keywords

Obésité ; Tissu adipeux ; Noradrénaline ; Lipolyse ; Tissu souscutané ; Adipocyte ; Mésentère ; Epiploon ; Récepteur hormonal ; Stimulant β-adrénergique ; Etude comparative ; Influence ; Homme ; Etat nutritionnel ; Trouble nutrition ; Catécholamine ; Hormone surrénalienne ;

Mots-clés espagnols / Spanish Keywords

Obesidad ; Tejido adiposo ; Noradrenalina ; Lipólisis ; Tejido subcutáneo ; Adipocito ; Mesenterio ; Epiplón ; Receptor hormonal ; Estimulante β-adrenérgico ; Estudio comparativo ; Influencia ; Hombre ; Estado nutricional ; Trastorno nutricíon ; Catecolamina ; Hormona suprarrenal ;

Localisation / Location

INIST-CNRS, Cote INIST : 18243, 35400006974159.0030