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Titre du document / Document title

A Novel Ca2+ Influx Pathway Activated by Mechanical Stretch in Human Airway Smooth Muscle Cells

Auteur(s) / Author(s)

ITO Satoru ; KUME Hiroaki ; NARUSE Keiji ; KONDO Masashi ; TAKEDA Naoya ; IWATA Susumu ; HASEGAWA Yoshinori ; SOKABE Masahiro ;

Résumé / Abstract

In response to mechanical stretch, airway smooth muscle exhibits various cellular functions such as contraction, proliferation, and cytoskeletal remodeling, all of which are implicated in the pathophysiology of asthma. We tested the hypothesis that mechanical stretch of airway smooth muscle cells increases intracellular Ca2+ concentration ([Ca2+]i) by activating stretch-activated (SA) non-selective cation channels. A single uniaxial stretch (3 s) was given to human bronchial smooth muscle cells cultured on an elastic silicone membrane. After the mechanical stretch, a transient increase in [Ca2+]i was observed. The [Ca2+]i increase was significantly dependent on stretch amplitude. The augmented [Ca2+]i due to stretch was completely abolished by removal of extracellular Ca2+ and was markedly attenuated by an application of Gd3+, an inhibitor of SA channels, or ruthenium red, a transient receptor potential vanilloid (TRPV) inhibitor. In contrast, the stretch-induced rises of [Ca2+]i were not altered by other Ca2+ channel inhibitors such as nifedipine, BTP-2, and SKF-96365. Moreover, the [Ca2+]i increases were not affected by indomethacin, a cyclooxygenase inhibitor, U-73122, a phospholipase C inhibitor, or xestospongin C, an inhibitor of the inositol-trisphosphate receptor. These findings demonstrate that a novel Ca2+ influx pathway activated by mechanical stretch, possibly through the Ca2+-permeable SA channel activated directly by stretch rather than by indirect mechanisms via intracellular messenger production, is involved in human airway smooth muscle cells. A molecular candidate for the putative SA channel may be one of the members of the TRPV channel family. Thus, abnormal Ca2+ homeostasis in response to excessive mechanical strain would contribute to the pathogenesis of asthma.

Revue / Journal Title

American journal of respiratory cell and molecular biology    ISSN  1044-1549   CODEN AJRBEL 

Source / Source

2008, vol. 38, no4, pp. 407-413 [7 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

American Thoracic Society, New York, NY, ETATS-UNIS  (1989) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 22187, 35400018331349.0060

Nº notice refdoc (ud4) : 20208028



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