Titre du document / Document title

Efficient dsRNA-mediated transgenic resistance to Beet necrotic yellow vein virus in sugar beets is not affected by other soilborne and aphid-transmitted viruses

Auteur(s) / Author(s)

LENNEFORS Britt-Louise ⁽¹⁾ ; VAN ROGGEN Petra M. ⁽¹⁾ ; YNDGAARD Flemming ⁽¹⁾ ; SAVENKOV Eugene I. ⁽²⁾ ; VALKONEN Jari P. T. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Syngenta Seeds AB, Box 302, 261 23 Landskrona, SUEDE
⁽²⁾ Department of Plant Biology and Forest Genetics, Swedish University of Agricultural Sciences, Box 7080, 750 07 Uppsala, SUEDE

Résumé / Abstract

Rhizomania caused by Beet necrotic yellow vein virus (BNYVV) is one of the most devastating sugar beet diseases. Sugar beet plants engineered to express a 0.4 kb inverted repeat construct based on the BNYVV replicase gene accumulated the transgene mRNA to similar levels in leaves and roots, whereas accumulation of the transgene-homologous siRNA was more pronounced in roots. The roots expressed high levels of resistance to BNYVV transmitted by the vector, Polymyxa betae. Resistance to BNYVV was not decreased following co-infection of the plants with Beet soil borne virus and Beet virus Q that share the same vector with BNYVV. Similarly, co-infection with the aphid-transmitted Beet mild yellowing virus, Beet yellows virus (BYV), or with all of the aforementioned viruses did not affect the resistance to BNYVV, while they accumulated in roots. These viruses are common in most of the sugar beet growing areas in Europe and world wide. However, there was a competitive interaction between BYV and BMYV in sugar beet leaves, as infection with BYV decreased the titres of BMYV. Other interactions between the viruses studied were not observed. The results suggest that the engineered resistance to BNYVV expressed in the sugar beets of this study is efficient in roots and not readily compromised following infection of the plants with heterologous viruses.

Revue / Journal Title

Transgenic research   ISSN 0962-8819 

Source / Source

2008, vol. 17, n^o2, pp. 219-228 [10 page(s) (article)] (2 p.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1991) (Revue)

Mots-clés anglais / English Keywords

Virus ; Benyvirus ; Invertebrata ; Arthropoda ; Insecta ; Homoptera ; Aphidoidea ; Aphididae ; Soils ; Beet necrotic yellow vein virus ; Gene silencing ; Aphis ; Sugar beet ; Resistance ; Transgenesis ;

Mots-clés français / French Keywords

Virus ; Benyvirus ; Invertebrata ; Arthropoda ; Insecta ; Homoptera ; Aphidoidea ; Aphididae ; Sol ; Virus nervure jaune nécrotique betterave ; Silence expression génique ; Aphis ; Betterave sucrière ; Résistance ; Transgenèse ;

Mots-clés espagnols / Spanish Keywords

Virus ; Benyvirus ; Invertebrata ; Arthropoda ; Insecta ; Homoptera ; Aphidoidea ; Aphididae ; Suelo ; Beet necrotic yellow vein virus ; Silencio expresión genética ; Aphis ; Remolacha azucarera ; Resistencia ; Transgénesis ;

Mots-clés d'auteur / Author Keywords

Aphis-borne viruses ; engineered resistance ; gene silencing ; rhizomania ; soil-borne viruses ; sugar beet ;

Localisation / Location

INIST-CNRS, Cote INIST : 26051, 35400018368572.0060