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Titre du document / Document title

Cytotoxicity of fig fruit latex against human cancer cells

Auteur(s) / Author(s)

JING WANG (1) ; XIUJIE WANG (1) ; SHU JIANG (2) ; PING LIN (1) ; JIE ZHANG (1) ; YANRONG LU (1) ; QI WANG (1) ; ZHUJUAN XIONG (1) ; YAYING WU (1) ; JINGJING REN (1) ; HONGLIANG YANG (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Division of Experimental Oncology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, CHINE
(2) Neourosurgery Department, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, CHINE

Résumé / Abstract

Fig fruit latex (FFL) contains significant amounts of polyphenolic compounds and can serve as a source of antioxidants after human consumption. The purpose of this study is to confirm anticancer activity of FFL against human cancer cells and to further elucidate its mechanism of activity. Human glioblastoma, hepatocellular carcinoma, and normal liver cells were used for in vitro tests of FFL effects. Those tests included cytotoxicity, colony formation inhibition, Brdu incorporation, acridine orange/ethidium bromide (AO/EB) staining for apoptotic cells, cell cycle distribution through flow cytometry (FCM), and ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 1 (ADPERL1) mRNA expression through RT-PCR in response to FFL treatment. After FFL treatment, the proliferation, colony formation, and Brdu labeling indices of cancer cells decreased (P < 0.05), while the AO/EB stained apoptotic cells increased (P < 0.05). By FCM analysis, an increase of G0)/G1 phase cell population and decrease of S and G2/M phase cells were observed (P < 0.01), while both ADPRTL1 mRNA expression and apoptotic indices increased (P < 0.01). The findings in these studies suggested that FFL exhibited potent cytotoxicity in some human cancer cells with little effect in normal cells at certain concentration. The mechanism for such effects might be associated with the inhibition of DNA synthesis, induction of apoptosis, and cell cycle arrest of cancer cells.

Revue / Journal Title

Food and chemical toxicology    ISSN  0278-6915   CODEN FCTOD7 

Source / Source

2008, vol. 46, no3, pp. 1025-1033 [9 page(s) (article)] (1 p.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Oxford, ROYAUME-UNI  (1982) (Revue)

Mots-clés anglais / English Keywords

Feeding

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Euphorbiaceae

;

Food

;

Cell death

;

Apoptosis

;

Inhibitor

;

Inhibition

;

Growth

;

Tumor cell

;

Human

;

Hevea brasiliensis

;

Natural rubber

;

Latex

;

Nutrition

;

Diet

;

Fruit

;

Cytotoxicity

;

Mots-clés français / French Keywords

Alimentation

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Euphorbiaceae

;

Aliment

;

Mort cellulaire

;

Apoptose

;

Inhibiteur

;

Inhibition

;

Croissance

;

Cellule tumorale

;

Homme

;

Hevea brasiliensis

;

Caoutchouc naturel

;

Latex

;

Nutrition

;

Régime alimentaire

;

Fruit

;

Cytotoxicité

;

Mots-clés espagnols / Spanish Keywords

Alimentación

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Euphorbiaceae

;

Alimento

;

Muerte celular

;

Apoptosis

;

Inhibidor

;

Inhibición

;

Crecimiento

;

Célula tumoral

;

Hombre

;

Hevea brasiliensis

;

Caucho natural

;

Látex

;

Nutrición

;

Régimen alimentario

;

Fruto

;

Citotoxicidad

;

Mots-clés d'auteur / Author Keywords

Fig fruit latex

;

Human cancer cells

;

Growth inhibition

;

Apoptosis

;

Localisation / Location

INIST-CNRS, Cote INIST : 10616, 35400018322249.0210

Nº notice refdoc (ud4) : 20169480



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