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Titre du document / Document title

The Effect of the Volatile Oil from Ginger Rhizomes (Zingiber officinale), its Fractions and Isolated Compounds on the 5-HT3 Receptor Complex and the Serotoninergic System of the Rat Ileum

Auteur(s) / Author(s)

Riyazi A. (1) ; Hensel A. ; Bauer K. ; Geißler N. ; Schaaf S. ; Verspohl E. J. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Pharmacology, Institute of Pharmaceutical and Medicinal Chemistry, University of Muenster, Germany

Résumé / Abstract

A contribution of the volatile oil from ginger rhizomes ( ZINGIBER OFFICINALE Roscoe) on inhibiting the 5-HT 3 receptor complex had been shown. In the present study a possible interaction of some compounds of the volatile oil with the 5-HT 3 receptor system expressed in N1E-115 cells and with the serotoninergic system of the rat ileum was investigated. The volatile oil was obtained by steam distillation and fractionated using a silica gel column resulting in five fractions. Compounds of the fractions were identified by GC-MS. The influence of the volatile oil, its fractions and pure components on serotonin-induced [ 14C]guanidinium influx into N1E-115 cells was measured indicating the inhibitory interaction with the 5-HT 3 receptor channel system. Most potent inhibitors of cation influx were the volatile oil, fraction 4, β-pinene, terpinolene, α-copaene and α-phellandrene. The volatile oil and fractions 1 and 4 were not able to significantly influence either serotonin (10 μM)-induced maximum contraction of the rat ileum or the second phase of the biphasic contraction 2.5 min after serotonin addition. However, β-pinene, terpinolene and α-phellandrene reduced both contractions. In conclusion, the volatile oil and distinct compounds such as terpinolene, β-pinene and α-phellandrene interact with 5-HT 3 receptor channel system and possess an antispasmodic effect at the rat ileum. GC:gas chromatography MS:mass spectrometry LRI:linear retention index NIST:National Institute for Safety and Technology, USA cpm:counts per minute 5-HT:5-hydroxytryptamine, serotonin 2-Me-5-HT:2-Methyl-5-hydroxytryptamine, 5-HT 3 receptor agonist

Revue / Journal Title

Planta medica    ISSN  1439-0221 

Source / Source

2007, vol. 73, no4, pp. 355-362 [8 page(s) (article)]

Langue / Language

Anglais
Revue : Multilingue

Editeur / Publisher

Thieme, Stuttgart, ALLEMAGNE  (1999) (Revue)

Mots-clés d'auteur / Author Keywords

Ginger

;

5-HT3 receptor

;

volatile oil

;

rat ileum

;

N1E-115 cells

;

ß-pinene

;

terpinolene

;

α-copaene

;

α-phellandrene

;

Localisation / Location

35400060008118.0009

Tous droits réservés © Georg Thieme Verlag Stuttgart, New York, 2007
Nº notice refdoc (ud4) : 19930835



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