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Titre du document / Document title

Bacterial phylogenetic diversity in a constructed wetland system treating acid coal mine drainage

Auteur(s) / Author(s)

NICOMRAT Duongruitai (1) ; DICK Warren A. (1 2) ; DOPSON Mark (3) ; TUOVINEN Olli H. (1 4) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Environmental Science Graduate Program, The Ohio State University, Columbus, OH 43210, ETATS-UNIS
(2) School of Environment and Natural Resources-Soil Science, The Ohio State University, Wooster, OH 44691, ETATS-UNIS
(3) Department of Molecular Biology, Umed University, 901 87 Umed, SUEDE
(4) Department of Microbiology, The Ohio State University, Columbus, OH 43210, ETATS-UNIS

Résumé / Abstract

Microorganisms in acid mine drainage are typically acidophiles that mediate the oxidation of reduced compounds of iron and sulfur. However, microbial populations in wetland systems constructed to treat acid mine drainage are not well characterized. The purpose of this study was to analyze bacterial diversity, using cultivation-independent molecular ecological techniques, in a constructed wetland that received acid drainage from an abandoned underground coal mine. DNA was purified from Fe(III)-precipitates from the oxidized surface zone of wetland sediments and 16S rRNA gene sequences were amplified and cloned. A total of 200 clones were analyzed by restriction fragment length polymorphism (RFLP) and 77 unique RFLP patterns were obtained with four restriction enzymes. Of these patterns, 30 most dominant unique clones were selected for sequencing of their 16S rRNA genes. Half of these 30 clones could be matched with autotrophic iron- and sulfur-oxidizing bacteria (Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans). Several clones also formed a clade with heterotrophic iron-oxidizing bacteria (TRA2-10, TRA3-20, and TRA5-3) and heterotrophic bacteria (Stenotrophomas maltophilia, Bordetella spp., Alcaligenes sp., Alcaligenes faecalis, and Alcaligenes xylosoxidans). Approximately 40% and 35% of the analyzed RFLP restriction patterns were consistent with A. ferrooxidans and A. thiooxidans, respectively. The relatively high frequency of acidithiobacilli is consistent with the chemical and physical characteristics of this site-i.e., continuous, abundant supply of reduced iron and sulfur compounds, pH 3-4, ambient temperature, and limited organics originating from the coal seam and from vegetation or soil surrounding the inlet channel to the wetland. The RFLP results were consistent with our previous culture-independent PCR-DGGE and FISH study, showing relatively low bacterial diversity and predominance of mesophilic acidithiobacilli in oxic wetland sediments.

Revue / Journal Title

Soil biology & biochemistry    ISSN  0038-0717   CODEN SBIOAH 

Source / Source

2008, vol. 40, no2, pp. 312-321 [10 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1969) (Revue)

Mots-clés anglais / English Keywords

Soils

;

Constructed wetland

;

Soil science

;

Microbiology

;

Mine soil

;

Bacteria

;

Wetland

;

Restriction fragment length polymorphism

;

Acidophily

;

Drainage

;

Coal mine

;

Acid medium

;

Lagooning

;

Phylogeny

;

Biodiversity

;

Mots-clés français / French Keywords

Sol

;

Zone humide artificielle

;

Science du sol

;

Microbiologie

;

Terrain minier

;

Bactérie

;

Zone humide

;

Polymorphisme longueur fragment restriction

;

Acidophilie

;

Drainage

;

Mine charbon

;

Milieu acide

;

Lagunage

;

Phylogenèse

;

Diversité biologique

;

Mots-clés espagnols / Spanish Keywords

Suelo

;

Zona húmeda artificial

;

Ciencia del suelo

;

Microbiología

;

Terreno minero

;

Bacteria

;

Zona húmeda

;

Polimorfismo longitud fragmento restricción

;

Acidofilia

;

Drenaje

;

Mina carbón

;

Medio ácido

;

Lagunaje

;

Filogénesis

;

Diversidad biológica

;

Mots-clés d'auteur / Author Keywords

Acid coal mine drainage

;

Acidithiobacillus

;

Acidophilic bacteria

;

RFLP analysis

;

Wetland microbiology

;

Localisation / Location

INIST-CNRS, Cote INIST : 14360, 35400016215023.0040

Nº notice refdoc (ud4) : 19861520



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