Titre du document / Document title

Determination of acetaminophen, caffeine, and butalbital in a commercial tablet dosage form by micellar electrokinetic chromatography

Auteur(s) / Author(s)

HAQUE A. ⁽¹⁾ ; STEWART J. T. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, Athens, GA 30602-2352, ETATS-UNIS

Résumé / Abstract

A micellar electrokinetic chromatography (MEKC) method was developed to analyze acetaminophen (AP), caffeine (CF), and butalbital (BB) simultaneously in a commercial tablet dosage form. Uncoated fused silica capillary (70 cm x 50 μm i.d, 50 cm to detector) was used for the separation at an applied voltage of 20 kV. A phosphate run buffer (pH 9, 0.05 M) containing 0.05 M sodium dodecyl sulphate was used for analysis. Separations of the analytes were achieved within 12 min at ambient temperature with detection set at 220 nm. Calibration curves were prepared for AP (260-520 μg/mL), CF (30-64 μg/mL) and BB (40-80 μg/mL) by the internal standard method with methyl-p-hydroxybenzoate as internal standard. Correlation coefficients of calibration curves were greater than 0.990. A five second hydrodynamic injection was used for analysis. Tablet powder equivalent to one tablet weight was extracted with absolute methanol in an ultrasonic bath for 30 min. The methanol extract was diluted with water and injected into the CE system for quantitation. Recoveries of the analytes were in the 95-103% range.

Revue / Journal Title

Journal of liquid chromatography & related technologies   ISSN 1082-6076   CODEN JLCTFC 

Source / Source

1999, vol. 22, n^o14, pp. 2159-2166 (11 ref.)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Colchester, ROYAUME-UNI  (1996) (Revue)

Mots-clés anglais / English Keywords

Chemical analysis ; Quantitative analysis ; Multicomponent analysis ; Simultaneous measurement ; Paracetamol ; Caffeine ; Butalbital ; Dosage form ; Tablet ; Micellar electrokinetic capillary chromatography ; Ultraviolet detector ;

Mots-clés français / French Keywords

Analyse chimique ; Analyse quantitative ; Analyse multiélément ; Mesure simultanée ; Paracétamol ; Caféine ; Butalbital ; Forme pharmaceutique ; Comprimé ; Chromatographie MECC ; Détecteur UV ;

Mots-clés espagnols / Spanish Keywords

Análisis químico ; Análisis cuantitativo ; Análisis multielemento ; Medición simultánea ; Paracetamol ; Cafeína ; Butalbital ; Forma farmacéutica ; Tableta ; Cromatografía MECC ; Detector UV ;

Localisation / Location

INIST-CNRS, Cote INIST : 18068, 35400008595705.0050