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Titre du document / Document title

Anti-estrogenic activities of indole-3-carbinol in Cervical cells : Implication for prevention of Cervical cancer

Auteur(s) / Author(s)

YUAN F. (1) ; CHEN D.-Z. (1) ; LIU K. (1) ; SEPKOVIC D. W. (2) ; BRADLOW H. L. (2) ; AUBORN K. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Long Island Jewish Medical Center, The Long Island Campus for Albert Einstein College of Medicine, Department of Otolaryngology, New Hyde Park, NY 11040, ETATS-UNIS
(2) Strang Cancer Research Laboratory, New York, New York 10021, ETATS-UNIS

Résumé / Abstract

Background: Cervical cancer constitutes the second most common cancer in women. Estrogen promotes development of cervical cancer in cells infected with high risk human papillomaviruses (HPVs). We asked whether the phytochemical indole-3-carbinol (13C) has anti-estrogenic activities in cervical cells with the goal of preventing cancer in HPV infected cells. Materials and Methods: Using the cervical cancer cell line CaSki, we evaluated expression of HPV and cytochrome p450 (CYP) enzymes by Northern, RNase protection or quantitative RT-PCR. 13C binding to estrogen receptor was measured by competition with estradiol. Estrogen metabolites were measured by gas chromarography-mass spectrometry (GC-MS). Results: Estradiol increased expression of HPV oncogenes whereas 13C and the estrogen metabolite 2-hydroxyestrone (2-OHE) abrogated the estrogen-increased expression of HPV oncogenes. Both 13C and 2-OHE competed with estradiol for estrogen receptor binding. 13C enhanced gene expression of CYP enzymes responsible for 2-hydroxylation of estrogen, and induced the formation of 2-OHE. Conclusion: 13C has anti-estrogenic activities which should prevent cancer in cervical cells.

Revue / Journal Title

Anticancer research    ISSN  0250-7005 

Source / Source

Congrès
International Conference of Anticancer Research No6, Kallithea, Halkidiki , GRECE (21/10/1998)
1999, vol. 19, no3A, pp. 1673-1680 (38 ref.)

Langue / Language

Anglais

Editeur / Publisher

International Institute of Anticancer Research, Attiki, GRECE  (1980) (Revue)

Mots-clés anglais / English Keywords

Carcinoma

;

Uterine cervix

;

Human

;

Female

;

Biological activity

;

Anticarcinogen

;

Antiestrogen

;

Antihormone

;

Feeding

;

Cruciferae

;

Plant origin

;

Extract

;

In vitro

;

Established cell line

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Female genital diseases

;

Uterine cervix diseases

;

Malignant tumor

;

Mots-clés français / French Keywords

Carcinome

;

Col utérus

;

Homme

;

Femelle

;

Activité biologique

;

Anticarcinogène

;

Antioestrogène

;

Antihormone

;

Alimentation

;

Cruciferae

;

Origine végétale

;

Extrait

;

In vitro

;

Lignée cellulaire établie

;

Indole-3-carbinol

;

Lignée MCF 7

;

Lignée CaSki

;

Lignée C33 A

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Appareil génital femelle pathologie

;

Col utérus pathologie

;

Tumeur maligne

;

Mots-clés espagnols / Spanish Keywords

Carcinoma

;

Cuello útero

;

Hombre

;

Hembra

;

Actividad biológica

;

Anticarcinógeno

;

Antiestrógeno

;

Antihormona

;

Alimentación

;

Cruciferae

;

Origen vegetal

;

Extracto

;

In vitro

;

Línea celular establecida

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Aparato genital hembra patología

;

Cuello útero patología

;

Tumor maligno

;

Localisation / Location

INIST-CNRS, Cote INIST : 19426, 35400008613920.0080

Nº notice refdoc (ud4) : 1940476



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