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Titre du document / Document title

Anti-allergic properties of a new all-D synthetic immunoglobulin-binding peptide

Auteur(s) / Author(s)

ROSSI Maria ; RUVO Menotti ; MARASCO Daniela ; COLOMBO Maurizio ; CASSANI Giovanni ; VERDOLIVA Antonio ;

Résumé / Abstract

Using a combinatorial chemistry approach, we identified a tetrameric tripeptide, denoted Protein A Mimetic (PAM) or TG19318, able to bind to immunoglobulins of different classes and species. The inverso variant, with the tripeptide in the all-D configuration (D-PAM or TG19320), is described as retaining binding properties to Ig. This peptide has now been assayed as a binder for E class immunoglobulins, in linear and competitive ELISA experiments, dot-blot and surface plasmon resonance (SPR) analyses. We show that D-PAM binds IgE with high specificity and selectivity, the interaction being sufficient to inhibit anaphylactic release of p-hexosaminidase from RBL 2H3 cells, with an IC50 value of 10 μg/mL. Intradermal administration of D-PAM suppresses PCA in the rat, with an IC50 of 1.25 μg/kg dose of peptide, while its intraperitoneal injection inhibits mouse PCA with an IC50of about 7 mg/kg and an efficacy comparable to that of ketotifen. Similarly, D-PAM inhibits ACA in the mouse, with 50% suppression at 10 mg/kg. The results presented here show that the peptide is active on the studied models, with effective doses below toxicity level, hence the molecule is a promising candidate for development of a new class of anti-allergic drugs.

Revue / Journal Title

Molecular immunology    ISSN  0161-5890   CODEN MOIMD5 

Source / Source

2008, vol. 45, no1, pp. 226-234 [9 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1979) (Revue)

Mots-clés d'auteur / Author Keywords

IgE

;

Allergic disorders

;

Passive cutaneous anaphylaxis

;

Active cutaneous anaphylaxis

;

Localisation / Location

INIST-CNRS, Cote INIST : 12239, 35400016164908.0250

Nº notice refdoc (ud4) : 19056222



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