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Titre du document / Document title

Effects of ethanol extracts from adzuki bean (Phaseolus angularis Wight.) and lima bean (Phaseolus lunatus L.) on estrogen and progesterone receptor phenotypes of MCF-7/BOS cells

Auteur(s) / Author(s)

ZHAO Qing-Wei (1 2) ; XIN HUANG (1) ; LOU Yi-Jia (1) ; WEBER Nadine (3) ; PROKSCH Peter (3) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Pharmacology and Toxicology, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310031, CHINE
(2) Department of Pharmacy, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, CHINE
(3) Institut für Pharmazeutische Biologie und Biotechnologie, Heinrich -Heine -Universtität Düsseldorf, 40225, Düsseldorf, ALLEMAGNE

Résumé / Abstract

In this study, the possible molecular mechanisms involved in the estrogen-like activities of ethanol extracts from two common Chinese legumes, i.e. Adzuki bean (Phaseolus angularis Wight.) and Lima bean (Phaseolus lunatus L.) were investigated. Reverse transcription-polymerase chain reaction (RT-PCR) measurement and western blot analysis were conducted to detect the functional mRNA and protein expressions, while immunofluorescence was used to determine the estrogen receptor (ER) intracellular distribution in the MCF-7/BOS cell line. The results indicated that (1) both mRNA and protein levels of progesterone receptor (PR) in the cell line increased after 48 h treatment with the extracts compared with those in the control; (2) Adzuki and Lima bean extracts might alter the ER intracellular distributions in two different manners: for the Adzuki bean, the ER appeared in both cytoplasm and nucleus, while for the Lima bean, it exhibited the same pattern as that of estradiol in which the ER almost disappeared in the nucleus and mainly occurred in the cytoplasm. Above all, the results showed a novel pattern of ER intracellular distribution in the MCF-7/BOS cell line upon exposure to Adzuki bean extract, suggesting that different pathways were involved in the estrogen-like activities of the ethanol extracts of Adzuki bean and Lima bean.

Revue / Journal Title

PTR. Phytotherapy research    ISSN  0951-418X 

Source / Source

2007, vol. 21, no7, pp. 648-652 [5 page(s) (article)] (3/4 p.)

Langue / Language

Anglais

Editeur / Publisher

Wiley, Chichester, ROYAUME-UNI  (1987) (Revue)

Mots-clés anglais / English Keywords

Vegetable crop

;

Grain legume

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Plant origin

;

Medicinal plant

;

Pharmacognosy

;

Established cell line

;

In vitro

;

Phenotype

;

Progesterone receptor

;

Estrogen receptor

;

Hormonal receptor

;

Phaseolus lunatus

;

Vigna angularis

;

Extract

;

Ethanol

;

Mots-clés français / French Keywords

Plante légumière

;

Légumineuse grain

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Origine végétale

;

Plante médicinale

;

Pharmacognosie

;

Lignée cellulaire établie

;

In vitro

;

Phénotype

;

Récepteur progestérone

;

Récepteur oestrogène

;

Récepteur hormonal

;

Phaseolus lunatus

;

Vigna angularis

;

Extrait

;

Ethanol

;

Mots-clés espagnols / Spanish Keywords

Hortalizas (plantas)

;

Leguminosa grano

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Origen vegetal

;

Planta medicinal

;

Farmacognosia

;

Línea celular establecida

;

In vitro

;

Fenotipo

;

Receptor progesterona

;

Receptor estrógeno

;

Receptor hormonal

;

Phaseolus lunatus

;

Vigna angularis

;

Extracto

;

Etanol

;

Mots-clés d'auteur / Author Keywords

ethanol extracts of Adzuki bean

;

ethanol extracts of Lima bean

;

MCF-7/BOS cell line

;

estrogen receptor

;

progesterone receptor

;

Localisation / Location

INIST-CNRS, Cote INIST : 21695, 35400014651880.0100

Nº notice refdoc (ud4) : 18877239



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