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Titre du document / Document title

New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway

Auteur(s) / Author(s)

YU Man-Shan (1) ; WONG Anita Yuk-Yee (1) ; SO Kwok-Fai (1 2 3) ; FANG Ji-Nian (4) ; YUEN Wai-Hung (5) ; CHANG Raymond Chuen-Chung (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Laboratory of Neurodegenerative Diseases, Department of Anatomy, The University of Hong Kong, Pokfulam, HONG-KONG
(2) Research Centre of Heart, Brain, Hormone and Healthy Aging, Faculty of Medicine, The University of Hong Kong, Pokfulam, HONG-KONG
(3) Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Potefulam, HONG-KONG
(4) Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhang Jiang Hi-Tech Park, Shanghai, CHINE
(5) Department of Chemistry and Open Laboratory of Chemical Biology of the Institute of Molecular Technology for Drug Discovery and Synthesis, The University of Hong Kong, Pokfulam, HONG-KONG

Résumé / Abstract

Most of the polysaccharides purified from Chinese medicinal herbs showed anti-tumor and immune-stimulating effects. However, little is known about their effects on neuroprotection. Our previous study has demonstrated that polysaccharides (J2, J3 and J4) isolated from the flowers of Nerium indicum (Oleander) exert partial protection in cortical neurons stressed by beta-amyloid (Aβ) peptides or deprivation of nutrition from serum. In this study, we have isolated and characterized a new polysaccharide from the flowers of Ν. indicum (named as J6) and aimed to investigate its neuroprotective effects against Aβ-induced apoptosis. Pretreatment of the polysaccharide J6 significantly decreased the activity of caspase-3 as well as the cytotoxicity triggered by Aβ peptides in a dose-dependent manner. In contrast to the activation of survival signaling such as Akt found in J2, J3 and J4 fractions, neuroprotective effects of J6 markedly inhibited Aβ peptide-stimulated phosphorylation of c-Jun N-terminal kinase (JNK-1) as determined by Western blot analysis. Taken together, the polysaccharide J6 isolated from the flowers of N. indicum can serve as potential neuroprotective agent against neuronal death in Alzheimer's disease and the neuroprotective mechanism may primarily rely on inactivation of JNK signaling pathway.

Revue / Journal Title

Brain research    ISSN  0006-8993   CODEN BRREAP 

Source / Source

2007, vol. 1153, pp. 221-230 [10 page(s) (article)] (1 p.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1966) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Apocynaceae

;

Enzyme

;

Transferases

;

Nerium oleander

;

Plant origin

;

β Amyloid protein

;

Neuroprotective agent

;

Polysaccharide

;

Amyloid

;

Signal transduction

;

Kinase

;

Stress

;

Neuron

;

Mots-clés français / French Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Apocynaceae

;

Enzyme

;

Transferases

;

Nerium oleander

;

Origine végétale

;

Protéine amyloïde β

;

Neuroprotecteur

;

Polyoside

;

Amyloïde

;

Transduction signal

;

Kinase

;

Stress

;

Neurone

;

Mots-clés espagnols / Spanish Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Apocynaceae

;

Enzima

;

Transferases

;

Nerium oleander

;

Origen vegetal

;

Proteína amiloide β

;

Neuroprotector

;

Poliósido

;

Amiloide

;

Transducción señal

;

Kinase

;

Estrés

;

Neurona

;

Localisation / Location

INIST-CNRS, Cote INIST : 12895, 35400016232663.0250

Nº notice refdoc (ud4) : 18808604



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