Titre du document / Document title
Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L
Auteur(s) / Author(s)
REDDY Muntha K. (1) ;
GUPTA Sashi K. (1) ;
JACOB Melissa R. (2) ;
KHAN Shabana I. (2) ;
FERREIRA Daneel (1 2) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department ofPharmacognosy, School of Pharmacy, The University of Mississippi, University MS, ETATS-UNIS
(2) National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University MS, ETATS-UNIS
Résumé / Abstract
The Punica granatum L (pomegranate) by-product POMx was partitioned between water, EtOAc and n-BuOH, and the EtOAc and n-BuOH extracts were purified by XAD-16 and Sephadex LH-20 column chromatography to afford ellagic acid (1), gallagic acid (2), punicalins (3), and punicalagins (4). Compounds 1-4 and the mixture of tannin fractions (XAD-16 eluates) were evaluated for antioxidant, antiplasmodial, and antimicrobial activities in cell-based assays. The mixture of tannins (TPT), XAD-EtOAc, XAD-H
2O, XAD-PJ and XAD-BuOH, exhibited IC
50 values against reactive oxygen species (ROS) generation at 0.8-19μg/ mL. Compounds 1-4 showed IC50values of 1.1, 3.2, 2.3 and 1.4 pM, respectively, against ROS generation and no toxicity up to 31.25 μg/mL against HL-60 cells. Gallagic acid (2) and punicalagins (4) exhibited antiplasmodial activity against Plasmodium falciparum D6 and W2 clones with IC50values of 10.9,10.6, 7.5 and 8.8 μM, respectively. Fractions XAD-EtOAc, XAD-BuOH, XAD-H
20 and XAD-PJ compounds 1-4 revealed antimicrobial activity when assayed against Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Cryptococcus neoformans, methicillin-resistant Staphylococcus aureus (MRSA),Aspergillus fumigatus and Mycobacterium intracellulare. Compounds 2 and 4 showed activity against P. aeruginosa, C. neoformans, and MRSA. This is the first report on the antioxidant, antiplasmodial and antimicrobial activities of POMx isolates, including structure-activity relationships (SAR) of the free radical inhibition activity of compounds 1-4. Our results suggest a beneficial effect from the daily intake of POMx and pomegranate juice (PJ) as dietary supplements to augment the human immune system's antioxidant, antimalarial and antimicrobial capacities.
Revue / Journal Title
Planta medica
ISSN
0032-0943
CODEN PLMEAA
Source / Source
2007, vol. 73, n
o5, pp. 461-467 [7 page(s) (article)] (30 ref.)
Langue / Language
Anglais
Editeur / Publisher
Thieme, Stuttgart, ALLEMAGNE
(1953)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 9624, 35400016225402.0080
Nº notice refdoc (ud4) : 18773247
