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Titre du document / Document title

Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L

Auteur(s) / Author(s)

REDDY Muntha K. (1) ; GUPTA Sashi K. (1) ; JACOB Melissa R. (2) ; KHAN Shabana I. (2) ; FERREIRA Daneel (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department ofPharmacognosy, School of Pharmacy, The University of Mississippi, University MS, ETATS-UNIS
(2) National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University MS, ETATS-UNIS

Résumé / Abstract

The Punica granatum L (pomegranate) by-product POMx was partitioned between water, EtOAc and n-BuOH, and the EtOAc and n-BuOH extracts were purified by XAD-16 and Sephadex LH-20 column chromatography to afford ellagic acid (1), gallagic acid (2), punicalins (3), and punicalagins (4). Compounds 1-4 and the mixture of tannin fractions (XAD-16 eluates) were evaluated for antioxidant, antiplasmodial, and antimicrobial activities in cell-based assays. The mixture of tannins (TPT), XAD-EtOAc, XAD-H2O, XAD-PJ and XAD-BuOH, exhibited IC50 values against reactive oxygen species (ROS) generation at 0.8-19μg/ mL. Compounds 1-4 showed IC50values of 1.1, 3.2, 2.3 and 1.4 pM, respectively, against ROS generation and no toxicity up to 31.25 μg/mL against HL-60 cells. Gallagic acid (2) and punicalagins (4) exhibited antiplasmodial activity against Plasmodium falciparum D6 and W2 clones with IC50values of 10.9,10.6, 7.5 and 8.8 μM, respectively. Fractions XAD-EtOAc, XAD-BuOH, XAD-H20 and XAD-PJ compounds 1-4 revealed antimicrobial activity when assayed against Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Cryptococcus neoformans, methicillin-resistant Staphylococcus aureus (MRSA),Aspergillus fumigatus and Mycobacterium intracellulare. Compounds 2 and 4 showed activity against P. aeruginosa, C. neoformans, and MRSA. This is the first report on the antioxidant, antiplasmodial and antimicrobial activities of POMx isolates, including structure-activity relationships (SAR) of the free radical inhibition activity of compounds 1-4. Our results suggest a beneficial effect from the daily intake of POMx and pomegranate juice (PJ) as dietary supplements to augment the human immune system's antioxidant, antimalarial and antimicrobial capacities.

Revue / Journal Title

Planta medica    ISSN  0032-0943   CODEN PLMEAA 

Source / Source

2007, vol. 73, no5, pp. 461-467 [7 page(s) (article)] (30 ref.)

Langue / Language

Anglais

Editeur / Publisher

Thieme, Stuttgart, ALLEMAGNE  (1953) (Revue)

Mots-clés anglais / English Keywords

Fruit tree

;

Phenols

;

Asia

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Punicaceae

;

Iran

;

China

;

Folk medicine

;

Plant origin

;

Medicinal plant

;

Pharmacognosy

;

Punica granatum

;

Phenolic acid

;

Tannin

;

Polyphenol

;

Antimicrobial agent

;

Parasiticide

;

Antimalarial

;

Antioxidant

;

Mots-clés français / French Keywords

Arbre fruitier

;

Phénols

;

Asie

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Punicaceae

;

Médecine traditionnelle persane

;

Médecine traditionnelle chinoise

;

Ellagitanin

;

Iran

;

Chine

;

Médecine traditionnelle

;

Origine végétale

;

Plante médicinale

;

Pharmacognosie

;

Punica granatum

;

Acide phénolique

;

Tanin

;

Polyphénol

;

Antimicrobien

;

Antiparasitaire

;

Antipaludique

;

Antioxydant

;

Mots-clés espagnols / Spanish Keywords

Arbol frutal

;

Fenoles

;

Asia

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Punicaceae

;

Iran

;

China

;

Medicina tradicional

;

Origen vegetal

;

Planta medicinal

;

Farmacognosia

;

Punica granatum

;

Acido fenólico

;

Tanino

;

Polifenol

;

Antimicrobiano

;

Antiparasitario

;

Antipalúdico

;

Antioxidante

;

Mots-clés d'auteur / Author Keywords

Punica granatum Punicaceae

;

ellagitannins

;

phenolic acids

;

antioxidant

;

antiplasmodial

;

antimicrobial activity

;

Localisation / Location

INIST-CNRS, Cote INIST : 9624, 35400016225402.0080

Nº notice refdoc (ud4) : 18773247



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