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Titre du document / Document title

Matrix effect and cross-reactivity of select amphetamine-type substances, designer analogues, and putrefactive amines using the bio-quant direct ELISA presumptive assays for amphetamine and methamphetamine

Auteur(s) / Author(s)

APOLLONIO Luigino G. (1) ; WHITTALL Ian R. (2) ; PIANCA Dennis J. (2) ; KYD Jennelle M. (1) ; MAHER William A. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) National Centre for Forensic Studies, University of Canberra, Bruce ACT 2601, AUSTRALIE
(2) Department of Toxicology and Forensic Chemistry, ACT Government Analytical Laboratory, Weston Creek ACT 2611, AUSTRALIE

Résumé / Abstract

The aim of this study was to evaluate the Bio-Quant Direct ELISA assays for amphetamine and methamphetamine in the routine presumptive screening of biological fluids. Standard concentration curves of the target analytes were assayed to assess sensitivity, and known concentrations of common amphetamine-type substances (ephedrine, pseudoephedrine, phentermine), designer analogues (MDA, MDMA, MDEA, MBDB, PMA, 4-MTA, 2CB), and putrefactive amines (phenylethylamine, putrescine, tryptamine, tyramine) were analyzed to determine cross-reactivity. Results of the standard curve studies show the capacity of both Direct ELISA kits to confidently detect down to 3 ng/mL interday (PBS matrix; CVs 6.3-15.5%). Cross-reactivity relative to that of 50 ng/mL preparations of the target compounds demonstrated that the Direct ELISA kit for amphetamine also detected MDA (282%), PMA (265%), 4-MTA (280%), and phentermine (61%), and the Direct ELISA for methamphetamine also assayed positive for MDMA (73%), MDEA (18%), pseudoephedrine (19%), MBDB (8%), and ephedrine (9%). Matrix studies demonstrated that both ELISA kits could be applied to screening of blood, urine, and saliva to a concentration of 6 ng/mL or lower. In conclusion, the Bio-Quant Direct ELISA kits for amphetamine and methamphetamine are fast and accurate and have demonstrated themselves to be useful tools in routine toxicological testing.

Revue / Journal Title

Journal of analytical toxicology    ISSN  0146-4760   CODEN JATOD3 

Source / Source

2007, vol. 31, no4, pp. 208-213 [6 page(s) (article)] (29 ref.)

Langue / Language

Anglais

Editeur / Publisher

Preston Publications, Niles, IL, ETATS-UNIS  (1977) (Revue)

Mots-clés anglais / English Keywords

Amphetamine derivatives

;

Chemical analysis

;

Biogenic amine

;

Drug of abuse

;

Enzyme immunoassay

;

CNS stimulant

;

Psychotropic

;

Biological fluid

;

Medical screening

;

Phentermine

;

Pseudoephedrine

;

Ephedrine

;

Cross reaction

;

Metamfetamine

;

ELISA assay

;

Polyamine

;

Amfetamine

;

Matrix effect

;

Mots-clés français / French Keywords

Amphétamine dérivé

;

Analyse chimique

;

Amine biogène

;

Substance toxicomanogène

;

Méthode immunoenzymatique

;

Stimulant SNC

;

Psychotrope

;

Liquide biologique

;

Dépistage

;

Phentermine

;

Pseudoéphédrine

;

Ephédrine

;

Réaction croisée

;

Métamfétamine

;

Technique ELISA

;

Polyamine

;

Amfétamine

;

Effet matrice

;

Mots-clés espagnols / Spanish Keywords

Amfetamina derivado

;

Análisis químico

;

Amina biógena

;

Sustancia toxicomanógena

;

Método inmunoenzimático

;

Estimulante SNC

;

Psicotropo

;

Líquido biológico

;

Descubrimiento

;

Fentermina

;

Pseudoefedrina

;

Reacción cruzada

;

Metanfetamina

;

Técnica ELISA

;

Poliamina

;

Anfetamina

;

Efecto matriz

;

Localisation / Location

INIST-CNRS, Cote INIST : 17415, 35400015991327.0050

Nº notice refdoc (ud4) : 18738346



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