Titre du document / Document title
Tandem mass spectrometry for sequencing proanthocyanidins
Auteur(s) / Author(s)
LI Hui-Jing (1) ;
DEINZER Max L. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Chemistry, Oregon State University, Corvallis, Oregon 97331, ETATS-UNIS
Résumé / Abstract
Proanthocyanidins (PAs) are a group of bioflavonoids consisting of oligomers based on catechin monomeric units. These polyphenolic compounds are widely distributed in higher plants and are an integral part of the human diet. A sensitive LC-tandem mass spectrometric (LC/ESI-MS
n) method in the positive ion mode for sequencing these ubiquitous and highly beneficial antioxidants is described. The hydroxylation patterns and interflavanoid linkage for A- and B-type PAs were determined by fragment ions derived from a retro-Diels-Alder (RDA) fission, heterocyclic ring fission (HRF), a novel benzofuran-forming (BFF) fission described here for the first time, and a quinone methide (QM) fission. The subunit sequence of the PAs was determined by diagnostic ions derived from HRF/RDA fission, HRF/BFF fission, and RDA/HRF fission together with QM fission. A total of 26 PAs were reliably sequenced by the newly established tandem mass spectrometric protocol. It is shown that the protocol based on a combination of these different fragmentation patterns allows for uniquely identifying PA oligomers.
Revue / Journal Title
Analytical chemistry
ISSN
0003-2700
CODEN ANCHAM
Source / Source
2007, vol. 79, n
o4, pp. 1739-1748 [10 page(s) (article)]
Langue / Language
Anglais
Editeur / Publisher
American Chemical Society, Washington, DC, ETATS-UNIS
(1947)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 120 B, 35400014554720.0640
Nº notice refdoc (ud4) : 18534021
