RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Investigation of cytotoxic activity on human cancer cell lines of arborinine and furanoacridones isolated from Ruta graveolens

Auteur(s) / Author(s)

RETHY Borbala (1) ; ZUPKO Istvan (1) ; MINORICS Renata (1) ; HOHMANN Judit (2) ; OCSOVSZKI Imre (3) ; FALKAY George (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, HONGRIE
(2) Department of Pharmacognosy, University of Szeged, Szeged, HONGRIE
(3) Department of Biochemistry, University of Szeged, Szeged, HONGRIE

Résumé / Abstract

The cytotoxic effects of a series of furanoacridones isolated from Ruta graveolens L. (Rutaceae) and of two further acridone alkaloids (arborinine and evoxanthine) were investigated by means of the MTT assay, using the human cell lines HeLa, MCF7 and A431. Arborinine proved best in inhibiting the proliferation of all three cell lines. The cytotoxic potency of the furacridone alkaloids was a function of their lipid solubility, which was determined by means of PAMPA. The capacity of the most effective furanoacridones to induce apoptosis was demonstrated by flow cytometric cell cycle analysis and by staining with ethidium bromide and acridine orange. This finding was reinforced by determining the apoptosis-regulating factors Bcl-2 and Bax, which were revealed by means of RT-PCR to change dose-dependently. The data presented here indicate that naturally occurring furanoacridones can be regarded as excellent starting structures for the potential development of new anticancer agents.

Revue / Journal Title

Planta medica    ISSN  0032-0943   CODEN PLMEAA 

Source / Source

2007, vol. 73, no1, pp. 41-48 [8 page(s) (article)] (30 ref.)

Langue / Language

Anglais

Editeur / Publisher

Thieme, Stuttgart, ALLEMAGNE  (1953) (Revue)

Mots-clés anglais / English Keywords

Essential oil plant

;

Ornamental shrub

;

Asia

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Iran

;

Folk medicine

;

Acridine derivatives

;

Plant origin

;

Medicinal plant

;

Pharmacognosy

;

Cell death

;

Apoptosis

;

Alkaloid

;

Rutaceae

;

In vitro

;

Established cell line

;

Tumor cell

;

Human

;

Cytotoxicity

;

Mots-clés français / French Keywords

Plante à huiles essentielles

;

Arbuste ornemental

;

Asie

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Médecine traditionnelle persane

;

Acridone dérivé

;

Ruta graveolens

;

Iran

;

Médecine traditionnelle

;

Acridine dérivé

;

Origine végétale

;

Plante médicinale

;

Pharmacognosie

;

Mort cellulaire

;

Apoptose

;

Alcaloïde

;

Rutaceae

;

Arborinine

;

In vitro

;

Lignée cellulaire établie

;

Cellule tumorale

;

Homme

;

Cytotoxicité

;

Mots-clés espagnols / Spanish Keywords

Planta de aceites esenciales

;

Arbusto ornamental

;

Asia

;

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Iran

;

Medicina tradicional

;

Origen vegetal

;

Planta medicinal

;

Farmacognosia

;

Muerte celular

;

Apoptosis

;

Alcaloide

;

Rutaceae

;

In vitro

;

Línea celular establecida

;

Célula tumoral

;

Hombre

;

Citotoxicidad

;

Mots-clés d'auteur / Author Keywords

Ruta graveolens L

;

Rutaceae

;

acridone alkaloids

;

cytotoxicity

;

apoptosis

;

Localisation / Location

INIST-CNRS, Cote INIST : 9624, 35400015933402.0060

Nº notice refdoc (ud4) : 18469419



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas