Titre du document / Document title
Investigation of cytotoxic activity on human cancer cell lines of arborinine and furanoacridones isolated from Ruta graveolens
Auteur(s) / Author(s)
RETHY Borbala (1) ;
ZUPKO Istvan (1) ;
MINORICS Renata (1) ;
HOHMANN Judit (2) ;
OCSOVSZKI Imre (3) ;
FALKAY George (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Pharmacodynamics and Biopharmacy, University of Szeged, Szeged, HONGRIE
(2) Department of Pharmacognosy, University of Szeged, Szeged, HONGRIE
(3) Department of Biochemistry, University of Szeged, Szeged, HONGRIE
Résumé / Abstract
The cytotoxic effects of a series of furanoacridones isolated from Ruta graveolens L. (Rutaceae) and of two further acridone alkaloids (arborinine and evoxanthine) were investigated by means of the MTT assay, using the human cell lines HeLa, MCF7 and A431. Arborinine proved best in inhibiting the proliferation of all three cell lines. The cytotoxic potency of the furacridone alkaloids was a function of their lipid solubility, which was determined by means of PAMPA. The capacity of the most effective furanoacridones to induce apoptosis was demonstrated by flow cytometric cell cycle analysis and by staining with ethidium bromide and acridine orange. This finding was reinforced by determining the apoptosis-regulating factors Bcl-2 and Bax, which were revealed by means of RT-PCR to change dose-dependently. The data presented here indicate that naturally occurring furanoacridones can be regarded as excellent starting structures for the potential development of new anticancer agents.
Revue / Journal Title
Planta medica
ISSN
0032-0943
CODEN PLMEAA
Source / Source
2007, vol. 73, n
o1, pp. 41-48 [8 page(s) (article)] (30 ref.)
Langue / Language
Anglais
Editeur / Publisher
Thieme, Stuttgart, ALLEMAGNE
(1953)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 9624, 35400015933402.0060
Nº notice refdoc (ud4) : 18469419
