Titre du document / Document title

Hexarelin-induced growth hormone response in short stature. Comparison with growth hormone-releasing hormone plus pyridostigmine and arginine plus estrogen

Auteur(s) / Author(s)

GUZZALONI G. ⁽¹⁾ ; GRUGNI G. ⁽¹⁾ ; MORABITO F. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Divisione di Auxologia, Ospedale San Giuseppe IRCCS, Istituto Auxologico Italiano, Verbania Intra, ITALIE

Résumé / Abstract

Hexarelin (HEX) is a synthetic hexapeptide with strong GH-stimulating activity. We evaluated GH response (expressed as maximum value after stimulus [C_max] and as area under the curve [AUC]) to HEX at the doses of 1 μg/kg iv (HEX 1) and 2 μg/kg iv (HEX 2), in comparison with the responses to GHRH (1 μg/kg iv) + pyridostigmine (PD, 60 mg po) and to arginine (ARG, 0.5 mg/kg iv) + ethinylestradiol (EE, 1 mg/day po for 3 days before the stimulation), in 5 subjects with familial short stature (FSS), 1 with constitutional growth delay (CGD), 6 with GH neurosecretory dysfunction (NSD), and 5 with isolated growth hormone deficiency (GHD). C_max and AUC after HEX 1 were 26.8±10.5 ng/ml and 1448±514 ng/min x ml in FSS, 23.6±14.4 ng/ml and 1146±750 ng/min x ml in CGD, 36.9±21.5 ng/ml and 2048±1288 ng/min x ml in NSD, 9.4±5.8 ng/ml and 498±200 ng/min x ml in GHD (C_max and AUC in FSS and CGD, p<0.05 vs GHD). C_max and AUC after HEX 2 were 37.7±16 ng/ml and 1979±888 ng/min x ml in FSS, 32.5±16.2 ng/ml and 1613±237 ng/min x ml in CGD, 39.7±20.7 ng/ml and 2366±1569 ng/min xml in NSD, 13.4±4.2 ng/ml and 645±293 ng/min x ml in GHD (C_max in FSS, CGD and NSD p<0.01 vs GHD; AUC in NSD, p<05 vs GHD). C_max and AUC after GHRH±PD were 46.6±8.8 ng/ml and 3294±1031 ng/min x ml in FSS, 25.9±11.2 ng/ml and 1464±735 ng/min x ml in CGD, 38.8±21.7 ng/ml and 2428±1399 ng/min x ml in NSD, 8.4±6.2 ng/ml and 685±572 ng/min x ml in GHD (C_max and AUC in FSS, p<0.001 vs CGD and GHD; C_max in CGD and NSD, p<0.001 vs GHD). C_max and AUC after ARG+EE were 21.3±4.2 ng/ml and 1432±514 ng/min x ml in FSS, 14.8±10 ng/ml and 805±489 ng/min x ml in CGD, 22.2±12.8 ng/ml and 1199±309 ng/min x ml in NSD, 4.6±2.5 ng/ml and 247±191 ng/min x ml in GHD (C_max and AUC in FSS, CGD and NSD, p<0.01 vs GHD). Specificity was 62% for HEX 1 and 75% for HEX 2, GHRH+PD and ARG+EE. From a diagnostic point of view, HEX 1 + HEX 2 was the association with the largest percentage of false positives (20% in FSS, 27% in CGD and 33% in NSD), HEX 1+GHRH+PD resulted in 9% in CGD, while the combined use of HEX 1 or HEX 2 with GHRH+PD or ARG+EE and of GHRH+PD with ARG+EE did not show false positive responses. In conclusion: I) the most effective dose of HEX was 2 μg/kg iv; 2) HEX did not show more specificity than GHRH+PD and ARG+EE; 3) the association of GHRH+PD with ARG+EE could yield the best results at lower costs, confirming these tests as first-line tools in evaluating GH secretion.

Revue / Journal Title

Journal of endocrinological investigation   ISSN 0391-4097   CODEN JEIND7 

Source / Source

1999, vol. 22, n^o5, pp. 360-368 (53 ref.)

Langue / Language

Anglais

Editeur / Publisher

Kurtis, Milano, ITALIE  (1978) (Revue)

Mots-clés anglais / English Keywords

Growth retardation ; Somatotropin hormone ; Hexapeptide ; Hormone releasing factor ; Biological activity ; Hormonal regulation ; Comparative study ; STH-RH test ; Pyridostigmine bromide ; Ethinylestradiol ; Arginin test ; Italy ; Child ; Adolescent ; Europe ; Human ; Adenohypophyseal hormone ;

Mots-clés français / French Keywords

Retard staturopondéral ; STH ; Hexapeptide ; Facteur libération hormonale ; Activité biologique ; Régulation hormonale ; Etude comparative ; Test STH-RH ; Bromure de pyridostigmine ; Ethinylestradiol ; Test arginine ; Italie ; Enfant ; Adolescent ; Hexarelin ; Europe ; Homme ; Hormone adénohypophysaire ;

Mots-clés espagnols / Spanish Keywords

Retraso pondoestatural ; STH ; Hexapéptido ; Factor liberación hormonal ; Actividad biológica ; Regulación hormonal ; Estudio comparativo ; Prueba STH-RH ; Bromuro de piridostigmina ; Etinilestradiol ; Prueba arginina ; Italia ; Niño ; Adolescente ; Europa ; Hombre ; Hormona adenohipofisaria ;

Localisation / Location

INIST-CNRS, Cote INIST : 18833, 35400008536923.0070