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Titre du document / Document title

Development and application of an indirect ELISA test for the detection of antibodies to Mycoplasma crocodyli infection in crocodiles (Crocodylus niloticus)

Auteur(s) / Author(s)

DAWO Fufa (1) ; MOHAN Krishna (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Paraclinical Studies, Faculty of Veterinary Sciences, University of Zimbabwe, P.O. Box 167, Harare, ZIMBABWE

Résumé / Abstract

Non-availability of a standardized rapid serodiagnostic test for quick and accurate diagnosis of Mycoplasma crocodyli (M. crocodyli) infection in crocodiles was the underlining reason for conducting the present study. An indirect enzyme-linked immunosorbent assay (iELISA) for the detection of antibodies (Ab) to M. crocodyli infection in crocodile sera was developed using sonicated antigen (Ag) and anti-crocodile conjugate. The iELISA test was optimised with different reagents and at different steps. A cut-off value of percent positive greater than or equal to 53.47% resulted in an estimated sensitivity and specificity of 85.67 and 100%, respectively. The developed iELISA could be used for detection of Abs to M. crocodyli infection in crocodiles and may enable to understand the transmission of the disease.

Revue / Journal Title

Veterinary microbiology    ISSN  0378-1135   CODEN VMICDQ 

Source / Source

2007, vol. 119, no2-4, pp. 283-289 [7 page(s) (article)] (3/4 p.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1976) (Revue)

Mots-clés anglais / English Keywords

Vertebrata

;

Reptilia

;

Crocodilia

;

Bacteria

;

Mollicutes

;

Mycoplasmatales

;

Mycoplasmataceae

;

Veterinary

;

Microbiology

;

Antibody

;

Detection

;

ELISA assay

;

Crocodylus niloticus

;

Mycoplasma

;

Mots-clés français / French Keywords

Vertebrata

;

Reptilia

;

Crocodilia

;

Bactérie

;

Mollicutes

;

Mycoplasmatales

;

Mycoplasmataceae

;

Vétérinaire

;

Microbiologie

;

Anticorps

;

Détection

;

Technique ELISA

;

Crocodylus niloticus

;

Mycoplasma

;

Mots-clés espagnols / Spanish Keywords

Vertebrata

;

Reptilia

;

Crocodilia

;

Bacteria

;

Mollicutes

;

Mycoplasmatales

;

Mycoplasmataceae

;

Veterinario

;

Microbiología

;

Anticuerpo

;

Detección

;

Técnica ELISA

;

Crocodylus niloticus

;

Mycoplasma

;

Mots-clés d'auteur / Author Keywords

Mycoplasma crocodyli

;

Crocodile

;

Serodiagnostic test

;

iELISA

;

Localisation / Location

INIST-CNRS, Cote INIST : 16884, 35400014532098.0210

Nº notice refdoc (ud4) : 18445932



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