Titre du document / Document title

The effect of h₁ calponin expression on gallstone formation in pregnancy

Auteur(s) / Author(s)

DING YOUMING ⁽¹⁾ ; WANG BIN ⁽¹⁾ ; WANG WEIXING ⁽¹⁾ ; WANG BINGHUA ⁽²⁾ ; LUO RUOYU ⁽³⁾ ; CHENG BANGCHANG ⁽⁴⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, CHINE
⁽²⁾ Department of Molecular Biology and Biochemistry, Renmin Hospital of Wuhan University, Wuhan, CHINE
⁽³⁾ Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, CHINE
⁽⁴⁾ Department of Thoracic and Cardiovascular Surgery, Renmin Hospital of Wuhan University, Wuhan, CHINE

Résumé / Abstract

Objectives: To explore the effect of h₁ calponin mRNA expression on the biliary tract dynamics, and investigate the molecular mechanisms of gallstone formation in pregnancy. Methods: This study was carried out in Renmin Hospital of Wuhan University, and in the Department of Molecular Biology and Biochemistry, Wuhan University School of Medicine, Wuhan, China from July to December 2004. Thirty female guinea pigs were divided randomly into 3 groups, the nonpregnant group (n=10) (group A), the 30 days of pregnancy group (n=10) (group B), and the 60 days of pregnancy group (n=10) (group C). Animal models of pregnancy were established on pregnant group guinea pigs through feeding animals with one cage according to female versus male as 4:1. The total cholesterol (TC), total bilirubin (TBiL), total bile acid (TBA) in the bile and the serum estradiol (E₂), progesterone (Pg) levels were determined respectively. Expression levels of h₁ calponin mRNA in gallbladder smooth muscles and Oddi's Sphincter (OS) were evaluated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR). Results: The concentration of TC, TBiL and the serum E₂ and Pg were more significantly increasing in group C than that in the other 2 groups. However, the concentration of TBA decreased gradually from group A to group C. Up-regulation of h₁ calponin gene expression was observed in the gallbladder smooth muscles in group C, but converse in OS. Conclusion: The h₁ calponin might play an important role in inducing dysfunction of extrahepatic biliary tract, bile stasis in gallbladder and gallstone formation in pregnancy.

Revue / Journal Title

Saudi medical journal   ISSN 0379-5284   CODEN SAMJDI 

Source / Source

2006, vol. 27, n^o11, pp. 1661-1666 [6 page(s) (article)] (30 ref.)

Langue / Language

Anglais

Editeur / Publisher

Saudi Medical Journal, Riyadh, ARABIE SAOUDITE  (1979) (Revue)

Mots-clés anglais / English Keywords

Biliary tract disease ; Digestive diseases ; Asia ; China ; Tropical medicine ; Woman ; Human ; Female ; Pregnancy ; Formation ; Gallbladder ; Cholelithiasis ;

Mots-clés français / French Keywords

Voie biliaire pathologie ; Appareil digestif pathologie ; Asie ; Calponine ; Chine ; Médecine tropicale ; Femme ; Homme ; Femelle ; Gestation ; Formation ; Vésicule biliaire ; Lithiase vésiculaire ;

Mots-clés espagnols / Spanish Keywords

Vía biliar patología ; Aparato digestivo patología ; Asia ; China ; Medicina tropical ; Mujer ; Hombre ; Hembra ; Gestación ; Formación ; Vesícula biliar ; Litiasis vesicular ;

Localisation / Location

INIST-CNRS, Cote INIST : 19973, 35400015958946.0050