Titre du document / Document title

GC-MS confirmation of codeine, morphine, 6-acetylmorphine, hydrocodone, hydromorphone, oxycodone, and oxymorphone in urine

Auteur(s) / Author(s)

MEATHERALL R. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Biochemistry, St. Boniface General Hospital, 409 Taché Avenue, Winnipeg, Manitoba, R2H 2A6, CANADA

Résumé / Abstract

A procedure for the simultaneous confirmation of codeine, morphine, 6-acetylmorphine, hydrocodone, hydromorphone, oxycodone, and oxymorphone in urine specimens by gas chromatography-mass spectrometry (GC-MS) is described. After the addition of nalorphine and naltrexone as the two internal standards, the urine is hydrolyzed overnight with β-glucuronidase from E. coli. The urine is adjusted to pH 9 and extracted with 8% trifluoroethanol in methylene dichloride. After evaporating the organic, the residue is sequentially derivatized with 2% methoxyamine in pyridine, then with propionic anhydride. The ketone groups on hydrocodone, hydromorphone, oxycodone, oxymorphone, and naltrexone are converted to their respective methoximes. Available hydroxyl groups on the O₃ and O₆ positions are converted to propionic esters. After a brief purification step, the extracts are analyzed by GC-MS using full scan electron impact ionization. Nalorphine is used as the internal standard for codeine, morphine, and 6-acetylmorphine; naltrexone is used as the internal standard for the 6-keto-opioids. The method is linear to 2000 ng/mL for the 6-keto-opioids and to 5000 ng/mL for the others. The limit of quantitation is 25 ng/mL in hydrolyzed urine. Day-to-day precision at 300 and 1500 ng/mL ranged between 6 and 10.9%. The coefficients of variation for 6-acetylmorphine were 12% at both 30 and 150 ng/mL. A list of 38 other basic drugs or metabolites detected by this method is tabulated.

Revue / Journal Title

Journal of analytical toxicology   ISSN 0146-4760   CODEN JATOD3 

Source / Source

1999, vol. 23, n^o3, pp. 177-186 (24 ref.)

Langue / Language

Anglais

Editeur / Publisher

Preston, Niles, IL, ETATS-UNIS  (1977) (Revue)

Mots-clés anglais / English Keywords

Chemical analysis ; Quantitative analysis ; Qualitative analysis ; Multicomponent analysis ; Opiates ; Morphine ; Hydrocodone ; Hydromorphone ; Oxycodone ; Oxymorphone ; Narcotic analgesic ; Biological fluid ; Urine ; Derivatization ; Gas chromatography ; Coupled method ; Mass spectrometry ; Electron impact ; Ion trap ;

Mots-clés français / French Keywords

Analyse chimique ; Analyse quantitative ; Analyse qualitative ; Analyse multiélément ; Opiacés ; Codéine ; Morphine ; Hydrocodone ; Hydromorphone ; Oxycodone ; Oxymorphone ; Analgésique narcotique ; Liquide biologique ; Urine ; Dérivatisation ; Chromatographie phase gazeuse ; Méthode couplée ; Spectrométrie masse ; Impact électron ; Piège ion ; Hydroxylamine(O-méthyl) ; Propionique acide anhydride ;

Mots-clés espagnols / Spanish Keywords

Análisis químico ; Análisis cuantitativo ; Análisis cualitativo ; Análisis multielemento ; Opiados ; Morfina ; Hidrocodona ; Hidromorfona ; Oxicodona ; Oximorfona ; Analgésico narcotico ; Líquido biológico ; Orina ; Derivatisación ; Cromatografía fase gaseosa ; Método acoplado ; Espectrometría masa ; Impacto electrón ; Trampa ión ;

Localisation / Location

INIST-CNRS, Cote INIST : 17415, 35400008461676.0080