Titre du document / Document title

Methylotenera mobilis gen. nov., sp. nov., an obligately methylamine-utilizing bacterium within the family Methylophilaceae

Auteur(s) / Author(s)

KALYUZHNAYA Marina G. ⁽¹⁾ ; BOWERMAN Sarah ⁽²⁾ ; LARA Jimmie C. ⁽³⁾ ; LIDSTROM Mary E. ^{(1 3)} ; CHISTOSERDOVA Ludmila ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Chemical Engineering, University of Washington, Seattle, WA 98195, ETATS-UNIS
⁽²⁾ Department of Biology, University of Washington, Seattle, WA 98195, ETATS-UNIS
⁽³⁾ Department of Microbiology, University of Washington, Seattle, WA 98195, ETATS-UNIS

Résumé / Abstract

A novel obligate methylamine utilizer (strain JLW8^T), isolated from Lake Washington sediment, was characterized taxonomically. The isolate was an aerobic, Gram-negative bacterium. Cells were rod-shaped and motile by means of a single flagellum. Reproduction was by binary fission and no resting bodies were formed. Growth was observed within a pH range of 5-8·5, with optimum growth at pH 7·5. It utilized methylamine as a single source of energy, carbon and nitrogen. Methylamine was oxidized via methylamine dehydrogenase and formaldehyde was assimilated via the ribulose monophosphate cycle. The cellular fatty acid profile was dominated by C_16:0ω7c and C_16:0 and the major phospholipid was phosphatidylethanolamine. The DNA G+C content was 54 mol%. 16S rRNA gene sequence analysis indicated that the new isolate was closely related (97-98% similarity) to a broad group of sequences from uncultured or uncharacterized Betaproteobacteria, but only distantly related (93-96 % similarity) to known methylotrophs of the family Methylophilaceae. Strain JLW8^T (=ATCC BAA-1282^T=DSM 17540^T) is proposed as the type strain of a novel species in a new genus within the family Methylophilaceae, Methylotenera mobilis gen. nov., sp. nov.

Revue / Journal Title

International journal of systematic and evolutionary microbiology   ISSN 1466-5026 

Source / Source

2006, vol. 56 (12), pp. 2819-2823 [5 page(s) (article)] (26 ref.)

Langue / Language

Anglais

Editeur / Publisher

Society for General Microbiology, Reading, ROYAUME-UNI  (2000) (Revue)

Mots-clés anglais / English Keywords

Freshwater environment ; Lake sediments ; Methylamine ; New species ; Taxonomy ; New genus ; Bacteria ;

Mots-clés français / French Keywords

Methylotenera mobilis ; Milieu eau douce ; Sédiment lacustre ; Méthylamine ; Espèce nouvelle ; Systématique ; Genre nouveau ; Bactérie ;

Mots-clés espagnols / Spanish Keywords

Medio agua dulce ; Sedimento lacustre ; Metilamina ; Especie nueva ; Sistemática ; Género nuevo ; Bacteria ;

Localisation / Location

INIST-CNRS, Cote INIST : 9775, 35400014515101.0160