Titre du document / Document title

Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates

Auteur(s) / Author(s)

KALYUZHNAYA Marina G. ⁽¹⁾ ; DE MARCO Paolo ⁽²⁾ ; BOWERMAN Sarah ⁽³⁾ ; PACHECO Catarina C. ⁽²⁾ ; LARA Jimmie C. ⁽⁴⁾ ; LIDSTROM Mary E. ^{(1 4)} ; CHISTOSERDOVA Ludmila ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Chemical Engineering, University of Washington, Seattle, WA 98195, ETATS-UNIS
⁽²⁾ Instituto de Biologia Molecular e Celular, Universidade do Porto, R. Campo Alegre, 823, 4150-180 Porto, PORTUGAL
⁽³⁾ Department of Biology, University of Washington, Seattle, WA 98195, ETATS-UNIS
⁽⁴⁾ Department of Microbiology, University of Washington, Seattle, WA 98195, ETATS-UNIS

Résumé / Abstract

The taxonomic positions and phylogenetic relationships of two new methylotrophic isolates from Lake Washington (USA) sediment, FAM5^T and 500, and the previously described methylotrophic strain EHg5 isolated from contaminated soil in Estarreja (Portugal) were investigated. All three strains were facultative methylotrophs capable of growth on a variety of C₁ and multicarbon compounds. Optimal growth occurred at pH 7·5-8 and 30-37 °C. The major fatty acids were C_16:1 ω7c and C_16:0. The major quinone was ubiquinone Q8. Neither methanol dehydrogenase nor methanol oxidase activities were detectable in cells grown on methanol, suggesting an alternative, as-yet unknown, mechanism for methanol oxidation. The isolates assimilated C₁ units at the level of formaldehyde, via the serine cycle. The DNA G + C content of the strains ranged between 64 and 65 mol%. 1 6S rRNA gene sequence similarity between the three new isolates was 99-85-1 00 °/o, but was below 94 % with other members of the Betaproteobacteria, indicating that the isolates represent a novel taxon. Based on physiological, phenotypic and genomic characteristics of the three isolates, a new genus, Methyloversatilis gen. nov., is proposed within the family Rhodocyclaceae. The type strain of Methyloversatilis universalis gen. nov., sp. nov. is FAM5^T (=CCUG 52030^%=JCM 13912^%).

Revue / Journal Title

International journal of systematic and evolutionary microbiology   ISSN 1466-5026 

Source / Source

2006, vol. 56 (11), pp. 2517-2522 [6 page(s) (article)] (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Society for General Microbiology, Reading, ROYAUME-UNI  (2000) (Revue)

Mots-clés anglais / English Keywords

New species ; Isolate ; Methylotrophy ; Taxonomy ; New genus ; Bacteria ;

Mots-clés français / French Keywords

Espèce nouvelle ; Isolat ; Methylotrophie ; Systématique ; Genre nouveau ; Bactérie ;

Mots-clés espagnols / Spanish Keywords

Especie nueva ; Aislado ; Metilotrofía ; Sistemática ; Género nuevo ; Bacteria ;

Localisation / Location

INIST-CNRS, Cote INIST : 9775, 35400015899702.0040