Titre du document / Document title

Intake of ¹³C-4 creatine enables simultaneous assessment of creatine and phosphocreatine pools in human skeletal muscle by ¹³C MR spectroscopy

Auteur(s) / Author(s)

KAN H. E. ; VAN DER GRAAF M. ; KLOMP D. W. J. ; VLAK M. H. M. ; PADBERG G. W. ; HEERSCHAP A. ;

Résumé / Abstract

The feasibility of a novel method for the noninvasive and local assessment of creatine (Cr) and phosphocreatine (PCr) dynamics in human skeletal muscle based on ¹³C magnetic resonance (MR) spectroscopy is presented. A high dose of Cr, labeled at the guanidino C-4 position with ¹³C 11% enrichment, was administered orally to a human subject for 5 days. Using a surface coil, ¹³C MR spectra of the lower leg were acquired on a 1.5T MR system at regular time intervals during and after Cr supplementation. An almost twofold increase in the intensities of the resolved PCr and Cr ¹³C-4 signals was observed during this period. The slow decrease in these signals to normal values after supplementation reflects the slow daily turnover of Cr. The PCr/Cr ratio did not appear to change over the whole measurement period. During exercise of the leg, reversible changes in PCr and Cr signals were observed, reflecting conversion by the Cr kinase reaction.

Revue / Journal Title

Magnetic resonance in medicine    ISSN  0740-3194   CODEN MRMEEN 

Source / Source

2006, vol. 56, n^o5, pp. 953-957 [5 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Williams & Wilkins, Baltimore, MD, ETATS-UNIS  (1984) (Revue)

Mots-clés d'auteur / Author Keywords

PCr/TCr

;

magnetic resonance spectroscopy

;

in vivo

;

creatine supplementation

;

creatine turnover

;

Localisation / Location

INIST-CNRS, Cote INIST : 20644, 35400015882104.0020