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Titre du document / Document title

Composition of the bacterial flora in tonsilloliths

Auteur(s) / Author(s)

TSUNEISHI Midori (1) ; YAMAMOTO Tatsuo (1) ; KOKEGUCHI Susumu (2) ; TAMAKI Naofumi (1) ; FUKUI Kazuhiro (2) ; WATANABE Tatsuo (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, JAPON
(2) Depaitment of Oral Microbiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, JAPON

Résumé / Abstract

Tonsilloliths are a potential cause of oral malodor. In this study, microbial profiles and composition of tonsilloliths were determined using culture-independent molecular methods and scanning electron microscopy. 16S ribosomal RNA bacterial genes (16S rDNAs) isolated from tonsilloliths of 6 individuals were amplified by PCR and cloned into Escherichia coli. Partial 16S rDNA sequences of approximately 600 bases of cloned inserts were used to determine species identity by comparison with sequences of known species. Characteristics of bacteria on the surface and inside the tonsillolith were analyzed using scanning electron microscopy. Anaerobic bacteria detected in tonsilloliths belonged to the genera Eubacterium, Fusobacterium, Megasphaera, Porphyromonas, Prevotella, Selenomonas and Tannerella, all of which appear to be associated with production of volatile sulfur compounds. Electron microscopy revealed cocci and rods on the surface and rods predominating inside the tonsilloliths. These results support the tonsillolith as an origin of oral malodor.

Revue / Journal Title

Microbes and infection    ISSN  1286-4579 

Source / Source

2006, vol. 8, no9-10, pp. 2384-2389 [6 page(s) (article)] (18 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1999) (Revue)

Mots-clés anglais / English Keywords

Volatile compound

;

Bacteria

;

Mots-clés français / French Keywords

Composé volatil

;

Bactérie

;

Mots-clés espagnols / Spanish Keywords

Compuesto volátil

;

Bacteria

;

Mots-clés d'auteur / Author Keywords

Tonsillolith

;

Bacteria

;

Oral malodor

;

Volatile sulfur compound

;

Localisation / Location

INIST-CNRS, Cote INIST : 26816, 35400015878821.0070

Nº notice refdoc (ud4) : 18219139



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