Titre du document / Document title

Comparative immunomodulatory effect of scopoletin on tumoral and normal lymphocytes

Auteur(s) / Author(s)

GABRIELA MANUELE Maria ; FERRARO Graciela ; BARREIRO ARCOS Maria Laura ; LOPEZ Paula ; CREMASCHI Graciela ; ANESINI Claudia ;

Résumé / Abstract

Some coumarins possess enhancing effects on lymphocyte mitogen responsiveness. In this investigation, the activity of scopoletin, a coumarin that has been isolated from different plants and in this case specifically from T. cordata Mill., was evaluated. For this purpose, normal T lymphocytes and a hyperproliferative T lymphoma cell line were used. Scopoletin was found to exert a dual action on tumoral lymphocytes exhibiting both a cytostatic and a cytotoxic effect. These effects varied with the concentrations analysed and the time of cell incubation (EC₅₀: 251 ± 15 μg/ml) and were associated to the induction of apoptosis. Scopoletin induced cell proliferation on normal T lymphocytes (Proliferation stimulation index: 1 μg/ml scopoletin: 1.26±0.1; 10 μg/ml scopoletin: 3±0.25; 100 μg/ml scopoletin: 1.86±0.08); this stimulatory action was found to be due to the interaction with kinase C (PKC) protein. These results indicate that scopoletin could be a potential antitumoral compound to be used for cancer treatment.

Revue / Journal Title

Life sciences    ISSN  0024-3205   CODEN LIFSAK 

Source / Source

2006, vol. 79, n^o21, pp. 2043-2048 [6 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1973) (Revue)

Mots-clés d'auteur / Author Keywords

Scopoletin

;

Tilia cordata

;

Lymphoma BW 5147

;

T lymphocytes

;

Immunomodulatory activity

;

Localisation / Location

INIST-CNRS, Cote INIST : 10194, 35400015229876.0110