Titre du document / Document title

Survival of a probiotic, Lactobacillus casei strain Shirota, in the gastrointestinal tract : Selective isolation from faeces and identification using monoclonal antibodies

Auteur(s) / Author(s)

YUKI N. ⁽¹⁾ ; WATANABE K. ⁽¹⁾ ; MIKE A. ⁽¹⁾ ; TAGAMI Y. ⁽¹⁾ ; TANAKA R. ⁽¹⁾ ; OHWAKI M. ⁽¹⁾ ; MOROTOMI M. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, JAPON

Résumé / Abstract

Lactobacillus casei strain Shirota (LCS) is a probiotic bacterium used mpein the production of fermented milk products and lactic acid bacteria preparations. To investigate the survival of LCS in the gastrointestinal tract, we have developed a selective medium and specific monoclonal antibodies to isolate and identify this strain. Selective LLV agar medium was prepared by modifying LBS medium, a selective medium for lactobacilli, through the replacement of glucose with lactitol as a carbon source and vancomycin as a selective antibiotic. Culture in LLV agar medium followed by ELISA using monoclonal antibodies specific for LCS was able to detect the organism in faeces. Using this method, we studied the faecal recovery of LCS in individuals who drank 125 ml of fermented milk which contained 10¹⁰ live LCS for 3 days. The mean recovery was about 10⁷ live bacteria per gram of faeces, indicating that LCS survived transit through the gastrointestinal tract after ingestion of the fermented milk.

Revue / Journal Title

International journal of food microbiology   ISSN 0168-1605   CODEN IJFMDD 

Source / Source

1999, vol. 48, n^o1, pp. 51-57 (10 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1984) (Revue)

Mots-clés anglais / English Keywords

Lactobacillus casei ; Probiotic ; Digestive tract ; Monoclonal antibody ; Selective medium ; Analysis method ; ELISA assay ; Fermented product ; Dairy product ; Dairy industry ; Survival ; Lactobacillaceae ; Bacteria ;

Mots-clés français / French Keywords

Lactobacillus casei ; Probiotique ; Tube digestif ; Anticorps monoclonal ; Milieu sélectif ; Méthode analyse ; Technique ELISA ; Produit fermenté ; Produit laitier ; Industrie laitière ; Survie ; Lactobacillaceae ; Bactérie ;

Mots-clés espagnols / Spanish Keywords

Lactobacillus casei ; Probioticó ; Tubo digestivo ; Anticuerpo monoclonal ; Medio selectivo ; Método análisis ; Técnica ELISA ; Producto fermentado ; Producto lácteo ; Industria láctea ; Sobrevivencia ; Lactobacillaceae ; Bacteria ;

Localisation / Location

INIST-CNRS, Cote INIST : 20302, 35400008482722.0050