Titre du document / Document title

Dietary supplementation of selenomethionine reduces metastasis of melanoma cells in mice

Auteur(s) / Author(s)

LIN YAN ⁽¹⁾ ; YEE J. A. ⁽¹⁾ ; DONGHUA LI ⁽¹⁾ ; MCGUIRE M. H. ⁽²⁾ ; GRAEF G. L. ⁽³⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Biomedical Sciences, Creighton University School of Medicine, Omaha, NE 68178, ETATS-UNIS
⁽²⁾ Department of Surgery, Creighton University School of Medicine, Omaha, NE 68131, ETATS-UNIS
⁽³⁾ Department of Agronomy, University of Nebraska-Lincoin, Lincoln, NE 68583, ETATS-UNIS

Résumé / Abstract

The present study investigated the effect of dietary supplementation of selenomethionine on pulmonary metastasis of B16BL6 murine melanoma cells in C57BL/6 mice. Mice were assigned to four groups of 15 each. They were fed a basal AIN-93G diet and the basal diet supplemented with 2.5 ppm or 5 ppm selenium as selenomethionine or with 2.5 ppm selenium as selenite for two weeks before and after the intravenous injection of 0.5 x 10⁵ tumor cells. At necropsy, the number and size of tumors that developed in the lungs were determined. The number of mice that had ≥11 tumors was 13, 8, 8, and 6 (p<0.02 compared with the control), and the median number of lung tumors was 64, 14, 12 (p<0.05 compared with the control), and 8 (p<0.01 compared with the control) in the control group and the groups with 2.5 ppm and 5 ppm selenium as selenomethionine and 2.5 ppm selenium as selenite. Dietary supplementation of selenomethionine decreased tumor cross-sectional area and tumor volume compared with the controls. At the same dietary level, selenite had a greater inhibitory effect on tumor size than selenomethionine. These results demonstrate that dietary supplementation of selenomethionine reduced experimental metastasis of melanoma cells in mice and inhibited the growth of metastatic tumors that formed in the lungs. It is concluded that selenomethionine is an active form of selenium that reduces experimental metastasis.

Revue / Journal Title

Anticancer research   ISSN 0250-7005 

Source / Source

1999, vol. 19, n^o2A, pp. 1337-1342 (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

International Institute of Anticancer Research, Attiki, GRECE  (1980) (Revue)

Mots-clés anglais / English Keywords

Melanoma ; Established cell line ; Metastasis ; Bronchopulmonary ; Selenium compound ; Antineoplastic agent ; Anticarcinogen ; Chemotherapy ; Supplementation ; Treatment ; Antimetastatic agent ; Animal model ; Mouse ; Animal ; Rodentia ; Mammalia ; Vertebrata ; Malignant tumor ; Lung disease ; Bronchus disease ; Respiratory disease ;

Mots-clés français / French Keywords

Mélanome ; Lignée cellulaire établie ; Métastase ; Bronchopulmonaire ; Sélénium composé ; Anticancéreux ; Anticarcinogène ; Chimiothérapie ; Supplémentation ; Traitement ; Antimétastatique ; Modèle animal ; Souris ; Animal ; Sélénométhionine ; Lignée B16BL6 ; Rodentia ; Mammalia ; Vertebrata ; Tumeur maligne ; Poumon pathologie ; Bronche pathologie ; Appareil respiratoire pathologie ;

Mots-clés espagnols / Spanish Keywords

Melanoma ; Línea celular establecida ; Metástasis ; Broncopulmonar ; Selenio compuesto ; Anticanceroso ; Anticarcinógeno ; Quimioterapia ; Suplementación ; Tratamiento ; Antimetastásico ; Modelo animal ; Ratón ; Animal ; Rodentia ; Mammalia ; Vertebrata ; Tumor maligno ; Pulmón patología ; Bronquio patología ; Aparato respiratorio patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 19426, 35400008522857.0660