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Titre du document / Document title

Measurement of a genotoxic hydrazine, agaritine, and its derivatives by HPLC with fluorescence derivatization in the Agaricus mushroom and its products

Auteur(s) / Author(s)

NAGADKA Megumi Hamano ; NAGAOKA Hiroaki ; KONDO Kazunari ; AKIYAMA Hiroshi ; MAITANI Tamio ;

Résumé / Abstract

Agaricus blazei MURRILL mushroom products are sold as socalled health foods in Japan. However, a part of Agaricus is known to contain hydrazines. A sensitive and specific method for analyzing a genotoxic hydrazine, agaritine, and its derivatives was developed to assess the safety of Agaricus products. β-N-(y-L(+)-Glutamyl)-4-(hydroxymethyl)phenylhydrazine (agaritine, AGT), 4-hydrazinylbenzylalcohol (HMPH), 4-hydrazinyl-benzoic acid (CPH), 4-methylphenylhydrazine (MPH) and phenylhydrazine (PH) were converted to their corresponding fluorescent derivatives with 3,4-dihydro-6,7 dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-COCl) as the fluorescence derivatization reagent. The detection limits (S/N=3) for CPH, AGT, PH and MPH were 422, 45.3, 16.5 and 138fmol, respectively, in a 20 μl injection volume. Recoveries, achieved by adding known AGT amounts to the Agaricus sample and Agaricus products, ranged from 92.8 to 102%. By using this method which does not require partial purification of the Agaricus sample, the amounts of AGT in several types of foods were found to be 112-1836 μg/g dry weight.

Revue / Journal Title

Chemical and pharmaceutical bulletin    ISSN  0009-2363   CODEN CPBTAL 

Source / Source

2006, vol. 54, no6, pp. 922-924 [3 page(s) (article)]

Langue / Language


Editeur / Publisher

JAACC, Tokyo, JAPON  (1958) (Revue)

Mots-clés d'auteur / Author Keywords





fluorescence derivatization




3,4-dihydro-6,7dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-COC1)


Localisation / Location

INIST-CNRS, Cote INIST : 4123, 35400013895850.0340

Nº notice refdoc (ud4) : 17950755

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