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Titre du document / Document title

A simple high-performance liquid chromatography method for the determination of throat-burning oleocanthal with probated antiinflammatory activity inextra virgin olive oils

Auteur(s) / Author(s)

IMPELLIZZERI John (1) ; JIANMING LIN (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Firmenich Inc., P.O. Box 5880, Princeton, New Jersey 08543, ETATS-UNIS

Résumé / Abstract

A high-performance liquid chromatography (HPLC) method was developed to quantitatively analyze oleocanthal in extra virgin olive oils. Oleocanthal, a deacetoxy ligstroside aglycone, is known to be responsible for the backof the throat irritation of olive oils and to have probated antiinflamatory activity. Oleocanthal was isolated from small amounts of olive oil sample (1 g) by liquid-liquid extraction. Hexane-acetonitrile was found to be the best solvent system to extract oleocanthal from the oil matrix. The solvent extract was analyzed by reversed-phase HPLC with UV detection at 278 nm. Chromatogaphic separation of oleocanthal from other extracted compounds and of the two geometric isomers of oleocanthal was achieved by an elution gradient with acetonitrile and water. Both the external standard calibration curve and the internal standard calibration curve were established, and quantitation using both calibration curves gave essentially the same result. The reproducibility (RSD = 4.7%), recovery (>95%), and limit of quantitation (<1 μg/g) were also determined. Concentrations of oleacanthal in 10 selected throat-burning extra virgin olive oils were determined using the method (ranged from 22 to 190 μg/g) with external standard calibration.

Revue / Journal Title

Journal of agricultural and food chemistry    ISSN  0021-8561   CODEN JAFCAU 

Source / Source

2006, vol. 54, no9, pp. 3204-3208 [5 page(s) (article)] (12 ref.)

Langue / Language

Anglais

Editeur / Publisher

American Chemical Society, Washington, DC, ETATS-UNIS  (1953) (Revue)

Mots-clés anglais / English Keywords

Vegetable oil

;

Edible oil

;

Liquid

;

High performance

;

Olive oil

;

Virgin oil

;

Antiinflammatory agent

;

Method

;

HPLC chromatography

;

Mots-clés français / French Keywords

Huile végétale

;

Huile alimentaire

;

Liquide

;

Haute performance

;

Huile olive

;

Huile vierge

;

Antiinflammatoire

;

Méthode

;

Chromatographie HPLC

;

Mots-clés espagnols / Spanish Keywords

Aceite vegetal

;

Aceite alimentario

;

Líquido

;

Alto rendimiento

;

Aceite oliva

;

Aceite virgen

;

Antiinflamatorio

;

Método

;

Cromatografía HPLC

;

Mots-clés d'auteur / Author Keywords

Oleocanthal

;

antiinflammatory

;

throat irritation

;

high performance liquid chromatorgraphy

;

Localisation / Location

INIST-CNRS, Cote INIST : 7332, 35400011552941.0060

Nº notice refdoc (ud4) : 17803590



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