Titre du document / Document title

Immunosuppressive effects of rutaecarpine in female BALB/c mice

Auteur(s) / Author(s)

TAE WON JEON ⁽¹⁾ ; CHUN HUA JIN ⁽¹⁾ ; SANG KYU LEE ⁽¹⁾ ; IN HYE JUN ⁽¹⁾ ; GHEE HWAN KIM ⁽¹⁾ ; DONG JU LEE ⁽¹⁾ ; HYE GWANG JEONG ⁽²⁾ ; LEE Kyung-Bok ⁽³⁾ ; JAHNG Yurngdong ⁽¹⁾ ; TAE CHEON JEONG ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ College of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 712-749, COREE, REPUBLIQUE DE
⁽²⁾ College of Pharmacy, Chosun University, Gwangju 501-759, COREE, REPUBLIQUE DE
⁽³⁾ College of Medicine, Konyang University, Nonsan 320-711, COREE, REPUBLIQUE DE

Résumé / Abstract

Rutaecarpine is a major quinazolinocarboline alkaloid isolated from Evodia rutaecarpa. It was reported to possess a wide spectrum of pharmacological activities, such as vasodilation, antithrombosis, and anti-inflammation. In the present study, adverse effects of rutaecarpine on immune functions were determined in female BALB/c mice. Rutaecarpine had no effects on hepatotoxicity parameters in mice, as measured by serum activities of aminotransferases. Meanwhile, rutaecarpine significantly decreased the number of antibody-forming cells and caused weight decrease in spleen in a dose-dependent manner, when mice were administered with rutaecarpine at 10 mg/kg, 20 mg/kg, 40mg/kg or 80 mg/kg once intravenously. In addition, rutaecarpine administered mice exhibited reduced splenic cellularity, decreased numbers of total T cells, CD4⁺ cells, CD8⁺ cells, and B cells in spleen. IL-2, interferon-γ and IL-10 mRNA expressions were suppressed significantly by rutaecarpine treatment. The number of CD4⁺IL-2⁺ cells was reduced significantly following administration of mice with rutaecarpine. Furthermore, rutaecarpine caused the cell cycle arrest in Go + G₁ phase in a dose-dependent manner. Rutaecarpine caused significant inductions of hepatic cytochrome P450 (CYP) 1A, 2B, and 2E1 activities dose-dependently. In the splenic lymphocyte proliferation assay, rutaecarpine inhibited proliferation by LPS and Con A ex vivo, whereas it had no effects on in vitro proliferation. These results suggested that a single bolus intravenous injection of rutaecarpine from 20 mg/kg might cause immunosuppressive effects, and that rutaecarpine-induced immunosuppression might be mediated, at least in part, through the inhibition of cytokine production and cell cycle arrest in Go + G₁ phase, and caused possibly by mechanisms associated with metabolic activation.

Revue / Journal Title

Toxicology letters   ISSN 0378-4274   CODEN TOLED5 

Source / Source

2006, vol. 164, n^o2, pp. 155-166 [12 page(s) (article)] (37 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1977) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; Cytokine ; Cell cycle ; Antibody ; Mouse ; Animal ; Female ; Immunosuppressive agent ; Immunosuppression ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; Cytokine ; Cycle cellulaire ; Anticorps ; Souris ; Animal ; Femelle ; Immunodépresseur ; Immunodépression ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; Citoquina ; Ciclo celular ; Anticuerpo ; Ratón ; Animal ; Hembra ; Inmunodepresor ; Inmunodepresión ;

Mots-clés d'auteur / Author Keywords

Rutaecarpine ; Immunosuppression ; Antibody-forming cells ; Cell cycle arrest ; Cytokine ;

Localisation / Location

INIST-CNRS, Cote INIST : 15984 L, 35400015663306.0070