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Titre du document / Document title

Dynamic gene expression profiling of Escherichia coli in carbon source transition from glucose to acetate

Auteur(s) / Author(s)

OH Min-Kyu (1) ; CHA Mee-Jeong (1) ; LEE Sun-Gu (2) ; ROHLIN Lars (3) ; LIAO James C. (3) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, COREE, REPUBLIQUE DE
(2) Department of Chemical Engineering, Pusan National University, Busan 609-735, COREE, REPUBLIQUE DE
(3) Department of Chemical Engineering, University of California at Los Angeles, CA 90095, ETATS-UNIS

Résumé / Abstract

DNA microarray was used to study the transcription profiling of Escherichia coli adapting to acetate as a sole carbon source. Bacteria grown in glucose minimal media were used as a reference. The dynamic expression levels of 3,497 genes were monitored at seven time points during this adaptation. Among the central metabolic genes, the glycolytic and glucose phosphotransferase genes were repressed as the bacteria entered stationary phase, whereas the glyoxylate pathway, TCA cycle, and gluconeogenic genes were induced. Distinct induction or repression patterns were recognized among different pathway genes. For example, the repression of glycolytic genes and the induction of gluconeogenic ones started immediately after glucose was depleted. On the other hand, the regulation of the pentose phosphate pathway genes and glyoxylate genes gradually responded to the glucose depletion or was more related to growth in acetate. When the whole genome was considered, many of the CRP, FadR, and Cra regulons were immediately responsive to the glucose depletion, whereas the σs, Lrp, and IHF regulons were gradually responsive to the glucose depletion. The expression profiling also provided differential regulations between isoenzymes; for example, malic enzymes A (sfcA) and B (maeB). The expression profiles of three genes were confirmed with RT-PCR.

Revue / Journal Title

Journal of microbiology and biotechnology    ISSN  1017-7825 

Source / Source

2006, vol. 16, no4, pp. 543-549 [7 page(s) (article)] (27 ref.)

Langue / Language

Anglais

Editeur / Publisher

Korean Society for Applied Microbiology, Seoul, COREE, REPUBLIQUE DE  (1991) (Revue)

Mots-clés anglais / English Keywords

Biosensor

;

Bacteria

;

Enterobacteriaceae

;

DNA chip

;

Acetate

;

Glucose

;

Escherichia coli

;

Gene expression

;

Dynamics

;

Mots-clés français / French Keywords

Biodétecteur

;

Bactérie

;

Enterobacteriaceae

;

source carbone

;

Puce à DNA

;

Acétate

;

Glucose

;

Escherichia coli

;

Expression génique

;

Dynamique

;

Mots-clés espagnols / Spanish Keywords

Biodetector

;

Bacteria

;

Enterobacteriaceae

;

Pulga de DNA

;

Acetato

;

Glucosa

;

Escherichia coli

;

Expresión genética

;

Dinámica

;

Mots-clés d'auteur / Author Keywords

DNA microarray

;

gene expression profiling

;

carbon source

;

glucose

;

acetate

;

Escherichia coli

;

Localisation / Location

INIST-CNRS, Cote INIST : 26482, 35400015321087.0080

Nº notice refdoc (ud4) : 17743606



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