Titre du document / Document title
Transformation of Actinidia eriantha : A potential species for functional genomics studies in Actinidia
Auteur(s) / Author(s)
TIANCHI WANG (1) ;
YIDONG RAN (1) ;
ATKINSON Ross G. (1) ;
GLEAVE Andrew P. (1) ;
COHEN Dan (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) The Horticulture and Food Research Institute of New Zealand Ltd., Private Bag 92169, Auckland, NOUVELLE-ZELANDE
Résumé / Abstract
Protocols were developed for regeneration and Agrobacterium-mediated transformation of Actinidia eriantha Benth. A. eriantha has a number of features that make it a useful tool for functional genomics in Actinidia: the vines are relatively small and non-vigorous in nature, flowers form all over the vine including on lower axillary branches and the species flowers prolifically in greenhouse conditions. Flowering and fruiting of transgenic A. eriantha plants was obtained within 2 years of transformation in a containment greenhouse. GUS (β-glucuronidase) activity indicating stable expression of the uidA gene was observed in leaf, stem, root, petal and fruit tissues. Molecular evidence for incorporation of transgenes into the A. eriantha genome was obtained by PCR and DNA gel blot analysis. Inheritance of transgenic phenotypes was demonstrated in seedling progeny. Functional genomic studies in kiwifruit have been initiated using transgenic A. eriantha plants.
Revue / Journal Title
Plant cell reports
ISSN
0721-7714
CODEN PCRPD8
Source / Source
2006, vol. 25, n
o5, pp. 425-431 [7 page(s) (article)] (38 ref.)
Langue / Language
Anglais
Editeur / Publisher
Springer, Berlin, ALLEMAGNE
(1981)
(Revue)
Mots-clés anglais / English Keywords
Spermatophyta ;
Angiospermae ;
Dicotyledones ;
Actinidiaceae ;
Bacteria ;
Rhizobiaceae ;
Actinidia deliciosa ;
Transgenic plant ;
Agrobacterium ;
Kiwi(fruit) ;
Genetic transformation ;
Genomics ;
Mots-clés français / French Keywords
Spermatophyta ;
Angiospermae ;
Dicotyledones ;
Actinidiaceae ;
Bactérie ;
Rhizobiaceae ;
Actinidia deliciosa ;
Plante transgénique ;
Agrobacterium ;
Kiwi(fruit) ;
Transformation génétique ;
Génomique ;
Mots-clés espagnols / Spanish Keywords
Spermatophyta ;
Angiospermae ;
Dicotyledones ;
Actinidiaceae ;
Bacteria ;
Rhizobiaceae ;
Actinidia deliciosa ;
Planta transgénica ;
Agrobacterium ;
Kiwi(fruta) ;
Transformación genética ;
Genómica ;
Mots-clés d'auteur / Author Keywords
Actinidia eriantha ;
Agrobacterium-mediated transformation ;
Functional genomics ;
Kiwifruit ;
Localisation / Location
INIST-CNRS, Cote INIST : 18737, 35400014266507.0070
Nº notice refdoc (ud4) : 17737773
