Titre du document / Document title

An EST catalogue from the resurrection plant Selaginella lepidophylla reveals abiotic stress-adaptive genes

Auteur(s) / Author(s)

ITURRIAGA Gabriel ⁽¹⁾ ; CUSHMAN Mary Ann F. ⁽²⁾ ; CUSHMAN John C. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Centro de Investigación en Biotecnologia, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Mor. 62210, MEXIQUE
⁽²⁾ Depamnent of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557-0014, ETATS-UNIS

Résumé / Abstract

The resurrection plant Selaginella lepidophylla (Division Lycophyta) belongs to an ancient lineage of vascular plants that can withstand complete desiccation for years and be revived after only a few hours of rehydration. To better understand the molecular basis of dehydration tolerance, a complementary DNA library was constructed from S. lepidophylla microphyll fronds dehydrated for 2.5 h and used to generate an expressed sequence tag (EST) database. ESTs were obtained for 1046 clones representing 874 unique transcripts. Putative functions were assigned to 653 (62.4%) of these clones after comparison with protein databases, whereas 212 (20.2%) sequences having significant similarity to known sequences whose functions are unclear and 181 (17.3%) sequences having no similarity to known sequences. The S. lepidophylla ESTs were compared to the Selaginella moellendorffii EST database using the tBLASTX algorithm. Approximately 36-38% of the S. lepidophylla ESTs exhibited similarity to the S. moellendorffii ESTs, whereas 62-63% were unique to S. lepidophylla and S. moellendorffii, respectively. For those S. lepidophylla ESTs for which functional assignments could be made, the largest functional categories included photosynthesis (17%), followed by primary metabolism (14%) and disease/defense-related functions (11%). S. lepidophylla had a higher relative percentage of ESTs within the transporter, cell structure, secondary metabolism, molecular chaperones (i.e., heat shock proteins), and abiotic stress-related (i.e., late embryogenesis abundance proteins) functional categories than did S. moellendorffii. Thus, S. lepidophylla may serve as a rich genetic resource for the identification of novel genes associated with environmental stress and dehydration tolerance.

Revue / Journal Title

Plant science   ISSN 0168-9452   CODEN PLSCE4 

Source / Source

2006, vol. 170, n^o6, pp. 1173-1184 [12 page(s) (article)] (65 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier Science, Shannon, IRLANDE  (1985) (Revue)

Mots-clés anglais / English Keywords

Embryonic development ; Heat shock protein ; Secondary metabolism ; Photosynthesis ; Expressed sequence tag ; Complementary DNA ; Tolerance ; Gene ; Stress ;

Mots-clés français / French Keywords

Développement embryonnaire ; Protéine choc thermique ; Métabolisme secondaire ; Photosynthèse ; Marqueur EST ; DNA complémentaire ; Tolérance ; Gène ; Stress ;

Mots-clés espagnols / Spanish Keywords

Desarrollo embrionario ; Proteína choque térmico ; Metabolismo secundario ; Fotosíntesis ; Marcador EST ; DNA complementario ; Tolerancia ; Gen ; Estrés ;

Mots-clés d'auteur / Author Keywords

Dehydration ; Desiccation ; Abiotic stress ; ESTs ; Lycophyte ; Selaginella lepidophylla ;

Localisation / Location

INIST-CNRS, Cote INIST : 15982, 35400014269642.0190