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Titre du document / Document title

The pheromone androstenol (5α-androst-16-en-3α-ol) is a neurosteroid positive modulator of GABAA receptors

Auteur(s) / Author(s)

KAMINSKI Rafal M. ; MARINI Herbert ; ORTINSKI Pavel I. ; VICINI Stefano ; ROGAWSKI Michael A. ;

Résumé / Abstract

Androstenol is a steroidal compound belonging to the group of odorous 16-androstenes, first isolated from boar testes and also found in humans. Androstenol has pheromone-like properties in both animals and humans, but the molecular targets of its pheromonal activity are unknown. Androstenol is structurally similar to endogenous A-ring reduced neurosteroids that act as positive modulators of GABAA receptors. Here we show that androstenol has neurosteroid-like activity as a GABAA receptor modulator. In whole-cell recordings from cerebellar granule cells, androstenol (but not its 3/3-epimer) caused a concentration-dependent enhancement of GABA-activated currents (EC50, 0.4 μM in cultures; 1.4 μM in slices) and prolonged the duration of spontaneous and miniature inhibitory postsynaptic currents. Androstenol (0.1-1 μM) also potentiated the amplitude of GABA-activated currents in human embryonic kidney 293 cells transfected with recombinant α1β2γ2 and α2β2γ2 GABAAreceptors and, at high concentrations (10-300 μM), directly activated currents in these cells. Systemic administration of androstenol (30-50 mg/kg) caused anxiolytic-like effects in mice in the open-field test and elevated zero-maze and antidepressant-like effects in the forced swim test (5-10 mg/ kg). Androstenol, but not its 3β-epimer, conferred seizure protection in the 6-Hz electroshock and pentylenetetrazol models (ED50 values, 21.9 and 48.9 mg/kg, respectively). The various actions of androstenol in the whole-animal models are consistent with its activity as a GABAA receptor modulator. GABAA receptors could represent a target for androstenol as a pheromone, for which it is well suited because of high volatility and lipophilicity, or as a conventional hormonal neurosteroid.

Revue / Journal Title

The Journal of pharmacology and experimental therapeutics    ISSN  0022-3565   CODEN JPETAB 

Source / Source

2006, vol. 317, no2, pp. 694-703 [10 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

American Society for Pharmacology and Experimental Therapeutics, Bethesda, MD, ETATS-UNIS  (1909) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 548, 35400015660567.0280

Nº notice refdoc (ud4) : 17721714



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