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Titre du document / Document title

Enrichment of n-3 PUFA contents on triglycerides of fish oil by lipase-catalyzed trans-esterification under supercritical conditions

Auteur(s) / Author(s)

LIN Tsao-Jen (1) ; CHEN Shin-Wan (1) ; CHANG An-Chan (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Chemical Engineering, National Chung-Cheng University, Chia-Yi 621, TAIWAN, PROVINCE DE CHINE

Résumé / Abstract

The immobilized 1,3-regiospecific lipase, Mucor miehei, was employed to catalyze the trans-esterification reaction to enrich n-3 polyunsaturated fatty acid (n-3 PUFA) content of triglycerides (TG) in Menhaden oil under supercritical carbon dioxide (SCCO2). A supercritical reacting system with a syringe pump to pressurize CO2 to supercritical condition and a high-pressure liquid pump to transfer the substrate into the reactor is applied to carry out experiments. Through the saponification of Menhaden oil followed by urea inclusion, 80.1 wt% of n-3 PUFA can be concentrated with containing 29.4 wt% EPA and 41.8 wt% DHA. The profile of conversion shows a constant increase at beginning 5 h, but after which the conversion levels off. Pressure up to 103.4 bars has a significant positive effect on the conversion of n-3 PUFA onto TG. Further increasing pressure, the conversion rate decreases due to the transformation of the spatial structure of lipase and leads to deactivate. The enzyme exhibits a good performance and stability in the region of 323 K. Ethanol is used as a co-solvent and its maximal addition is 10 wt% of the total substrates. The optimal substrate molar ratio of TG to n-3 PUFA is about 1/4 by considering the TG inhibition. The conversion in SCCO2 appears 40 wt% higher than in n-hexane at ambient pressure after 5 h. The ping-pong bi-bi mechanism was proposed for the kinetic model of the reaction. The final rate equation can be simplified and expressed by a modified Michaelis-Menten equation. Based on the evaluated kinetic constants, there was 7.3% deviation of the total n-3 PUFA content between the experimental and the predicted data.

Revue / Journal Title

Biochemical engineering journal    ISSN  1369-703X 

Source / Source

Congrès
Young Asian Biochemical Engineer's Community YABEC. Symposium No10, Osaka City , JAPON (23/09/2004)
2006, vol. 29, no 1-2 (169 p.)  [Document : 8 p.] (39 ref.), pp. 27-34 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1998) (Revue)

Mots-clés anglais / English Keywords

Enzyme

;

Hydrolases

;

Esterases

;

Carboxylic ester hydrolases

;

Kinetics

;

Production

;

Supercritical state

;

Esterification

;

Triacylglycerol lipase

;

Fish oil

;

Triglyceride

;

Polyunsaturated fatty acid

;

Mots-clés français / French Keywords

Enzyme

;

Hydrolases

;

Esterases

;

Carboxylic ester hydrolases

;

Cinétique

;

Production

;

Etat supercritique

;

Estérification

;

Triacylglycerol lipase

;

Huile poisson

;

Triglycéride

;

Acide gras polyinsaturé

;

Mots-clés espagnols / Spanish Keywords

Enzima

;

Hydrolases

;

Esterases

;

Carboxylic ester hydrolases

;

Cinética

;

Producción

;

Estado supercrítico

;

Esterificación

;

Triacylglycerol lipase

;

Aceite pescado

;

Triglicérido

;

Acido graso poliinsaturado

;

Mots-clés d'auteur / Author Keywords

Supercritical fluid

;

n-3 Polyunsaturated fatty acid

;

Trans-esterification

;

Production kinetics

;

Enzyme technology

;

Localisation / Location

INIST-CNRS, Cote INIST : 26675, 35400014284542.0040

Nº notice refdoc (ud4) : 17652876



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