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Titre du document / Document title

Evaluation of two methods for ammonia extraction and analysis in silage samples

Auteur(s) / Author(s)

KOZLOSKI G. V. (1) ; SENGER C. C. D. (1) ; PEROTTONI J. (2) ; BONNECARREE SANCHEZ L. M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Animal Science Department, Universidade Federal de Santa Maria (UFSM), Campus Camobi, Santa Maria 97105-900, RS, BRESIL
(2) Chemical Department, UFSM, Campus Camobi, Santa Maria 97105-900, RS, BRESIL

Résumé / Abstract

Three experiments were carried out to evaluate two methods of ammonia extraction and analysis in corn silage samples. In the first experiment ammonia was extracted with a 2M KCl and measured either by a phenol-hypochlorite or a microdifusion method. Ammonia content of samples analysed by microdifusion were significantly higher (97.9 versus 61.2mg of NH3-N/100 g of dry matter) (P<0.05) than those obtained by phenol-hypochlorite method. In the second experiment, fluid extraction from silage samples by mechanical pressing followed by phenol-hypochlorite assay was compared to extraction with 2 M KCl followed by microdifusion analysis of ammonia. The last method also resulted in higher ammonia values in silages (110.3 versus 95.4 mg of NH3-N/100 g of dry matter) (P<0.05). In this experiment, the accuracy of methods was also evaluated. There were not differences between runs for any of them. A third experiment was carried out to evaluate ammonia recovery from tubes, which was near 1.0 in both methods. An additional experiment was conducted to evaluate if aerobic exposure could change pH values and ammonia content of silage samples. It was verified that pH increased linearly through a 24 h period (P<0.05), varying between 3.91 and 4.34, while the slope for ammonia contents was not different from zero. Although both, phenol-hypochlorite and microdifusion methods were similarly accurate, mechanical pressing extraction and phenol-hypochlorite determination is a more practical and specific method for ammonia analysis in silage samples. Parallel use of phenol-hypochlorite and microdifusion methods, however, allow to estimate the volatile amines content in silages. Ammonia is not lost from silages until 24 h after removal from the silo.

Revue / Journal Title

Animal feed science and technology    ISSN  0377-8401   CODEN AFSTDH 

Source / Source

2006, vol. 127, no3-4, pp. 336-342 [7 page(s) (article)] (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1976) (Revue)

Mots-clés anglais / English Keywords

Phenol

;

Phenols

;

Sample

;

Silage

;

Extraction

;

Ammonia

;

Analysis method

;

Evaluation

;

Mots-clés français / French Keywords

Phénol

;

Phénols

;

Echantillon

;

Ensilage

;

Extraction

;

Ammoniac

;

Méthode analyse

;

Evaluation

;

Mots-clés espagnols / Spanish Keywords

Fenol

;

Fenoles

;

Muestra

;

Ensilaje

;

Extracción

;

Amoníaco

;

Método análisis

;

Evaluación

;

Mots-clés d'auteur / Author Keywords

Ammonia

;

Analysis

;

Microdifusion

;

Phenol-hypochlorite silage

;

Localisation / Location

INIST-CNRS, Cote INIST : 17215, 35400014283536.0120

Nº notice refdoc (ud4) : 17617966



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