Titre du document / Document title

Antioxidant activity of Nelumbo nucifera (sacred lotus) seeds

Auteur(s) / Author(s)

RAI Sujay ⁽¹⁾ ; WAHILE Atul ⁽¹⁾ ; MUKHERJEE Kakali ⁽¹⁾ ; BISHNU PADA SAHA ⁽¹⁾ ; MUKHERJEE Pulok K. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, 188 Raja S C Mullick Road, Kolkata 700 032, INDE

Résumé / Abstract

Antioxidant activity of hydro alcoholic extract of Nelumbo nucifera seeds (HANN) was studied using in vitro and in vivo models. Total phenolic content in HANN was found to be 7.61±0.04% (w/w). Characteristic HPTLC fingerprints of HANN were also made using different solvent systems. The HANN exhibited strong free radical scavenging activity as evidenced by the low IC₅₀ values in both DPPH (1,1-diphenyl-2-picryl hydrazyl) (6.12±0.41 μg/ml) and nitric oxide (84.86±3.56 μg/ml) methods. The values were found to be less than those of rutin, the standard used. Acute toxicity of HANN was evaluated in Swiss Albino mice, no signs of toxicity were observed up to the oral dose of 1000 mg/kg body weight. Administration of HANN to Wistar rats at 100 and 200 mg/kg body weight for 4 days prior to carbon tetrachloride (CCl₄) treatment caused a significant dose dependent increase (p<0.05 top<0.001) in the level of superoxide dismutase (SOD) and catalase and a significant decrease (p<0.05 to p<0.001) in the level of thiobarbituric acid reactive substances (TBARS), when compared to CCl₄ treated control in both liver and kidney. These changes observed at 100 mg/kg body weight treatment were comparable to those observed for standard Vitamin E at 50 mg/kg treatment. Nelumbo nucifera seeds contain alkaloids, saponins, phenolics and carbohydrates. The results support significant antioxidant nature of HANN.

Revue / Journal Title

Journal of ethnopharmacology   ISSN 0378-8741   CODEN JOETD7 

Source / Source

2006, vol. 104, n^o3, pp. 322-327 [6 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1978) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Plant origin ; Medicinal plant ; Pharmacognosy ; Extract ; Nymphaeaceae ; Seeds ; Lotus ; Antioxidant ;

Mots-clés français / French Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Nelumbo nucifera ; Origine végétale ; Plante médicinale ; Pharmacognosie ; Extrait ; Nymphaeaceae ; Graine ; Lotus ; Antioxydant ;

Mots-clés espagnols / Spanish Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Origen vegetal ; Planta medicinal ; Farmacognosia ; Extracto ; Nymphaeaceae ; Semillas ; Lotus ; Antioxidante ;

Mots-clés d'auteur / Author Keywords

Antioxidant ; Nelumbo nucifera ; Nymphaeaceae: Seeds ; Hydro alcoholic extract ;

Localisation / Location

INIST-CNRS, Cote INIST : 18028, 35400015344477.0070