Titre du document / Document title

Comparison of an automated and point-of-care immunoassay to GC-MS for urine oxycodone testing in the clinical laboratory

Auteur(s) / Author(s)

HALLER Christine A. ⁽¹⁾ ; STONE Judith ⁽²⁾ ; BURKE Vicki ⁽²⁾ ; BRANCH Jean ⁽²⁾ ; CHEN Kathy ⁽²⁾ ; GROSS Susan ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Medicine and Laboratory Medicine, University of California, San Francisco, California, ETATS-UNIS
⁽²⁾ Clinical Laboratories, San Francisco General Hospital, San Francisco, California, ETATS-UNIS

Résumé / Abstract

OxyContin®, a controlled-release formulation of oxycodone, is increasingly abused. Monitoring patient compliance by urine drug testing may deter illegal diversion of OxyContin. Two urine immunoassays were evaluated with a 100 ng/mL cutoff for oxycodone. The Microgenics Corporation Oxycodone DRI on the Bayer ADVIA 1650 and a point-of-care (POC) immunoassay, Monitect Oxycodone POC from Branan Medical Corporation, were compared to gas chromatography-mass spectrometry (GC-MS) with a detection limit of 50 ng/mL free oxycodone. Between-day precision for DRI yielded coefficients of variation from 3.9% to 7.0% at 75 and 125 ng/mL. Fifty-two positive and 52 negative urines were tested. The DRI had a 100% agreement with GC-MS. Two positive specimens had free oxycodone < 50 ng/mL, but oxycodone metabolites, oxymorphone and oxycodone glucuronide > 100 ng/mL, were identified by GC-MS analysis. The POC assay had two false positives and 15 indeterminate (±) results. Codeine or hydrocodone was present in all but one of these samples. There was no interference with DRI from morphine, codeine, hydrocodone, hydromorphone, dihydrocodeine, or 6-monoacetyl morphine. Four-hundred and ninety urine samples were subsequently tested with DRI to estimate the oxycodone-positive rate at our hospital, and 47 (9.4%) were positive. The confirmation rate with CC-MS for free oxycodone, not including metabolites, was 93%. The Microgenics DRI offers good performance for oxycodone urine testing and is a better choice for the clinical laboratory than the POC assay. Confirmation of screened positive samples requires a method that can detect total oxycodone and oxymorphone.

Revue / Journal Title

Journal of analytical toxicology   ISSN 0146-4760   CODEN JATOD3 

Source / Source

2006, vol. 30, n^o2, pp. 106-111 [6 page(s) (article)] (12 ref.)

Langue / Language

Anglais

Editeur / Publisher

Preston, Niles, IL, ETATS-UNIS  (1977) (Revue)

Mots-clés anglais / English Keywords

Opiates ; Narcotic analgesic ; Biological fluid ; Mass spectrometry ; Coupled method ; Gas chromatography ; Interference ; Quantitative analysis ; Chemical analysis ; Oxycodone ; Urine ; Immunological method ; Automation ; Comparative study ;

Mots-clés français / French Keywords

Opiacés ; GC MS ; Analgésique narcotique ; Liquide biologique ; Spectrométrie masse ; Méthode couplée ; Chromatographie phase gazeuse ; Interférence ; Analyse quantitative ; Analyse chimique ; Oxycodone ; Urine ; Méthode immunologique ; Automatisation ; Etude comparative ;

Mots-clés espagnols / Spanish Keywords

Opiados ; Analgésico narcotico ; Líquido biológico ; Espectrometría masa ; Método acoplado ; Cromatografía fase gaseosa ; Interferencia ; Análisis cuantitativo ; Análisis químico ; Oxicodona ; Orina ; Método inmunológico ; Automatización ; Estudio comparativo ;

Localisation / Location

INIST-CNRS, Cote INIST : 17415, 35400015338545.0070