Titre du document / Document title
Therapeutic effect of a traditional Chinese medicine, ren-shen-yang-rong-tang (Japanese name: Ninjin'yoeito) on nitric oxide-mediated lung injury in a mouse infected with murine cytomegalovirus
Auteur(s) / Author(s)
TANAKA Kazuo (1) ;
SAWAMURA Sadaaki (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Laboratory of Infections Diseases, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, JAPON
Résumé / Abstract
The therapeutic effect of a traditional Chinese medicine ren-shen-yang-rong-tang (Japanese name: Ninjin'yoeito, NYT) on murine cytomegalovirus (MCMV)-associated pneumonitis was examined. In MCMV-pneumonitis, IFN-γ-induced nitric oxide (NO) mediates its pathogenesis. When mice, which had been infected with 0.2LD
50 of MCMV at 28 days previously, were intraperitoneally injected with anti-CD3 monoclonal antibody (mAb), MCMV-pneumonitis was induced in the lung, where high amounts of IFN-γ-producing cells thereafter accumulated, accompanied by an elevation in the NO level in the serum and abundant expression of inducible NO synthase (iNOS) mRNA, thus resulting in all mice eventually dying. When the mice were orally treated with NYT (1000 mg/kg/day) once on the day of mAb injection and once the day after, the expression level of iNOS-mRNA was suppressed and NO level in the serum decreased. The survival rate improved from 0% to 57.1%. The pathological findings of the lungs in the NYT-treated mice were comparable to those of the uninfected controls. In contrast, NYT itself did not affect either the ratio of IFN-γ-producing cells or MCMV titer. As a result, NYT had a therapeutic effect on MCMV-pneumonitis by decreasing the degree of inflammation mediated by the IFN-γ-induced NO. It is also interesting to note that only two oral administrations of NYT had a therapeutic effect on viral disease.
Revue / Journal Title
International immunopharmacology
ISSN
1567-5769
Source / Source
2006, vol. 6, n
o4, pp. 678-685 [8 page(s) (article)] (21 ref.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Kidlington, ROYAUME-UNI
(2001)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 27109, 35400013284907.0190
Nº notice refdoc (ud4) : 17569319
