Titre du document / Document title

Altered gene expression in human cells treated with the insecticide diazinon : Correlation with decreased DNA excision repair capacity

Auteur(s) / Author(s)

MANKAME T. ⁽¹⁾ ; HOKANSON R. ⁽¹⁾ ; FUDGE R. ⁽¹⁾ ; CHOWDHARY R. ⁽¹⁾ ; BUSBEE D. ^{(1 2)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, ETATS-UNIS
⁽²⁾ Department of Environmental and Industrial Health, School of Rural Public Health, TAMU Health Science Center, Texas A&M University, College Station, TX 77843, ETATS-UNIS

Résumé / Abstract

Many industrial and agricultural chemicals have steroid hormone agonist or antagonist activities and disrupt hormone-regulated gene expression. The widely-used agricultural insecticide, diazinon, was evaluated using MCF-7 cells - a breast cancer-derived, estrogen-dependent, human cell line - to examine the capacity of this chemical to alter steroid hormone-regulated gene expression. MCF-7 cells were treated with 30, 50, or 67 ppm of diazinon, and gene expression in treated cells was measured as mRNA levels in the cells compared to mRNA levels in untreated or estrogen-treated cells. DNA microarray analysis showed significant up- or down-regulation of a number of genes in treated cells compared to untreated cells. Of the 600 human genes on the chip utilized, specific genes with related functions were selected for additional consideration. Real time quantitative PCR (qrtPCR) completed to corroborate mRNA levels as a measure of specific gene expression, confirmed results obtained from analysis of the microarray data. The data show that ERCC5, encoding Xeroderma pigmentosum protein G (XPG), essential for DNA excision repair, and ribonucleotide reductase subunit M1 (RNRM1), encoding a gene necessary for providing the nucleotides needed for DNA repair, were down-regulated in cells treated with diazinon. These studies were designed to provide base-line data on the gene expression-altering capacity of a specific agricultural chemical, diazinon, and allow assessment of some of the potentially deleterious effects associated with exposure of human cells to diazinon.

Revue / Journal Title

Human & experimental toxicology   ISSN 0960-3271 

Source / Source

2006, vol. 25, n^o2, pp. 57-65 [9 page(s) (article)] (39 ref.)

Langue / Language

Anglais

Editeur / Publisher

Arnold, London, ROYAUME-UNI  (1990) (Revue)

Mots-clés anglais / English Keywords

Pesticides ; Organophosphorus compounds ; In vitro ; DNA chip ; Repair ; DNA ; Diazinon ; Insecticide ; Human ; Gene expression ; Toxicity ;

Mots-clés français / French Keywords

Pesticide ; Organophosphoré ; In vitro ; Puce à DNA ; Réparation ; DNA ; Diazinon ; Insecticide ; Homme ; Expression génique ; Toxicité ;

Mots-clés espagnols / Spanish Keywords

Plaguicida ; Organofosforado ; In vitro ; Pulga de DNA ; Reparación ; DNA ; Diazinon ; Insecticida ; Hombre ; Expresión genética ; Toxicidad ;

Mots-clés d'auteur / Author Keywords

DNA microarray analysis ; gene expression ; agricultural chemicals ; organophosphate insecticides ; real time PCR ;

Localisation / Location

INIST-CNRS, Cote INIST : 19290, 35400015341044.0020