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Titre du document / Document title

Screening the allergenic repertoires of wheat and maize with sera from double-blind, placebo-controlled food challenge positive patients

Auteur(s) / Author(s)

WEICHEL M. (1) ; VERGOOSSEN N. J. (1 2) ; BONOMI S. (3) ; SCIBILIA J. (4) ; ORTOLANI C. (4) ; BALLMER-WEBER B. K. (5) ; PASTORELLO E. A. (3) ; CRAMERI R. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Swiss Institute of Allergy and Asthma Research (SIAF), Davos Platz, SUISSE
(2) Academic Hospital, Maastricht, PAYS-BAS
(3) Allergy Center, 1st Division of General Medicine Ospedale Maggiore IRCSS, Departement of Internal Medicine, University of Milan, ITALIE
(4) Bizzozzero Division, Niguarda Ca'Granda Hospital, Milan, ITALIE
(5) University Hospital, Zurich, SUISSE

Résumé / Abstract

Background: Food allergy to wheat and maize is an increasing factor of deterioration of life quality, especially childhood and can, in rare cases, even induce anaphylaxis. Although omega-5 gliadin from wheat and maize lipid transfer protein have been characterized as major cereal allergens on the molecular level, the list of food allergens is far to be complete. Methods: To identify the IgE-binding repertoires of wheat and maize we screened respective cDNA libraries displayed on phage surface with sera from patients with a confirmed food allergy. The study included six patients with a positive double-blind, placebo-controlled food challenge (DBPCFC) to wheat, nine patients with a positive DBPCFC to maize, and six patients with anaphylactic reactions after ingestion of wheat. Results: The enriched sequences encoding IgE-binding proteins showed heterogeneous repertoires for both, wheat and maize. The selected wheat repertoire yielded 12, the maize repertoire 11 open reading frames. Among these we identified allergens belonging to already characterized allergens families, such as gliadin, profilin and beta-expansin. Besides, we found novel proteins with high cross-reactive potential, such as thioredoxins, as well as sequences that had so far not been related to cereal allergy at all. The IgE-binding capacity of some selected proteins was evaluated in vitro and cross-reactivity was demonstrated by competition ELISA. Conclusion: With regard to the heterogeneity of the characterized sequences as well as to the biochemical nature of the new allergens detected we conclude that wheat and maize-related food allergy is more complex than so far anticipated.

Revue / Journal Title

Allergy    ISSN  0105-4538   CODEN LLRGDY 

Source / Source

2006, vol. 61, no1, pp. 128-135 [8 page(s) (article)] (35 ref.)

Langue / Language

Anglais

Editeur / Publisher

Blackwell, Oxford, ROYAUME-UNI  (1978) (Revue)

Mots-clés anglais / English Keywords

Immunopathology

;

Immunology

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Recombinant protein

;

IgE

;

Phage display

;

Gene

;

Complementary DNA

;

Human

;

Placebo

;

Serum

;

Zea mays

;

Allergen

;

Corn

;

Wheat

;

Allergy

;

Immunologic repertoire

;

Medical screening

;

Provocation test

;

Mots-clés français / French Keywords

Immunopathologie

;

Immunologie

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Protéine recombinante

;

IgE

;

Technique phage display

;

Gène

;

DNA complémentaire

;

Homme

;

Placebo

;

Sérum

;

Zea mays

;

Allergène

;

Maïs

;

Blé

;

Allergie

;

Répertoire immunologique

;

Dépistage

;

Test provocation

;

Mots-clés espagnols / Spanish Keywords

Inmunopatología

;

Inmunología

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Proteína recombinante

;

IgE

;

Técnica phage display

;

Gen

;

DNA complementario

;

Hombre

;

Placebo

;

Suero

;

Zea mays

;

Alergeno

;

Maiz

;

Trigo

;

Alergia

;

Repertorio inmunologico

;

Descubrimiento

;

Prueba provocación

;

Mots-clés d'auteur / Author Keywords

cDNA phage surface display. IgE-binding repertoires

;

maize allergy

;

recombinant allergens

;

wheat allergy

;

Localisation / Location

INIST-CNRS, Cote INIST : 5264, 35400013466579.0180

Nº notice refdoc (ud4) : 17477565



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