RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

The persistence, dissemination, and visceralization tendency of Leishmania major in Syrian hamsters

Auteur(s) / Author(s)

SOLIMAN Maha F. M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Zoology Department, Faculty of Science, Suez Canal University, Ismailia, EGYPTE

Résumé / Abstract

I monitored the persistence, dissemination, and the possible visceralization tendency ofLeishmania major, the causative parasite of cutaneous leishmaniasis in North Africa and the Middle East in Syrian hamsters (Mesocricetus auratus). Hamsters were inoculated subcutaneously in the hind footpad, with 1 x 106 L. major metacyclic promastigotes and were sacrificed at months 1, 3, 6 and 10 post-infection (pi). Skin lesions, blood, spleens, livers and kidneys were screened by both Giemsa-stained smears and a polymerase chain reaction (PCR) for detection ofL. major amastigotes. A few weeks pi, 61.7% of the inoculated hamsters developed a cutaneous lesion only at the inoculation site, while 38.3% of them developed non-self-healed lesions at sites distant from the inoculation site. PCR identified all the positive stained smears as well as false-negative ones, indicating that PCR was more sensitive than stained smears. The results confirmed the dissemination and persistence ofL. major amastigotes in all tissues examined, except the kidneys, for a period extending to 10 months, only in those hamsters suffering from disseminated cutaneous lesions. Parasite DNA was detected in the bloods starting from the first month pi and starting from month 3 pi in the spleens and livers. Some, but not all, the animals with disseminated infections proved to be positive for parasite DNA in their organs. Persistence of the L. major amastigotes in the tissues differed from those of other species causing visceral diseases. These findings demonstrate a possible visceralization tendency for L. major previously recorded for L. tropica and L. mexicana.

Revue / Journal Title

Acta tropica    ISSN  0001-706X   CODEN ACTRAQ 

Source / Source

2006, vol. 97, no2, pp. 146-150 [5 page(s) (article)] (28 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Oxford, ROYAUME-UNI  (1944) (Revue)

Mots-clés anglais / English Keywords

Molecular biology

;

Vertebrata

;

Mammalia

;

Rodentia

;

Asia

;

Protozoa

;

Kinetoplastida

;

Viscus

;

Experimental disease

;

Tropical medicine

;

Tissue

;

Hamster

;

Animal

;

Syria

;

Leishmania major

;

Dissemination

;

Polymerase chain reaction

;

Smear

;

Mots-clés français / French Keywords

Biologie moléculaire

;

Vertebrata

;

Mammalia

;

Rodentia

;

Asie

;

Protozoa

;

Kinetoplastida

;

Viscère

;

Pathologie expérimentale

;

Médecine tropicale

;

Tissu

;

Hamster

;

Animal

;

Syrie

;

Leishmania major

;

Dissémination

;

Réaction chaîne polymérase

;

Frottis

;

Mots-clés espagnols / Spanish Keywords

Biología molecular

;

Vertebrata

;

Mammalia

;

Rodentia

;

Asia

;

Protozoa

;

Kinetoplastida

;

Víscera

;

Patología experimental

;

Medicina tropical

;

Tejido

;

Hamster

;

Animal

;

Siria

;

Leishmania major

;

Diseminación

;

Reacción cadena polimerasa

;

Frotis

;

Mots-clés d'auteur / Author Keywords

Leishmania major

;

Hamsters

;

Tissue smears

;

Polymerase chain reaction (PCR)

;

Visceralization

;

Dissemination

;

Localisation / Location

INIST-CNRS, Cote INIST : 3165, 35400013473120.0040

Nº notice refdoc (ud4) : 17432503



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas