Titre du document / Document title

Evidence for a local renin angiotensin system in primary cultured human preadipocytes

Auteur(s) / Author(s)

SCHLING P. ⁽¹⁾ ; MALLOW H. ⁽¹⁾ ; TRINDL A. ⁽¹⁾ ; LÖFFLER G. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, 93040 Regensburg, ALLEMAGNE

Résumé / Abstract

OBJECTIVE: To investigate whether human preadipocytes possess a complete functional renin angiotensin system. MEASUREMENTS: Gene expression of angiotensinogen, renin, renin binding protein, angiotensin converting enzyme (ACE) and angiotensin II (ang II) receptor type 1 in human preadipocytes; ACE protein and ang II production of human adipose tissue stromal cells differentiated or not in primary culture. RESULTS: All genes mentioned above were found to be expressed in human preadipocytes. ACE was translated into protein as detected by western blot. Ang II was secreted both by undifferentiated preadipocytes and immature adipocytes, and its production was significantly elevated in differentiated cells. CONCLUSIONS: Preadipocytes from human adipose tissue express a functional renin angiotensin system (RAS).

Revue / Journal Title

International journal of obesity   ISSN 0307-0565   CODEN IJOBDP 

Source / Source

1999, vol. 23, n^o4, pp. 336-341 (32 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing, Basingstoke, ROYAUME-UNI  (1977) (Revue)

Mots-clés anglais / English Keywords

Preadipocyte ; Adipose tissue ; Gene expression ; Renin angiotensin system ; Angiotensin II ; Cell culture ; In vitro ; Molecular biology ; Human ; Peptides ;

Mots-clés français / French Keywords

Préadipocyte ; Tissu adipeux ; Expression génique ; Système renin angiotensine ; Angiotensine II ; Culture cellulaire ; In vitro ; Biologie moléculaire ; Homme ; Peptide ;

Mots-clés espagnols / Spanish Keywords

Preadipocito ; Tejido adiposo ; Expresión genética ; Sistema renin angiotensina ; Angiotensina II ; Cultivo celular ; In vitro ; Biología molecular ; Hombre ; Péptido ;

Localisation / Location

INIST-CNRS, Cote INIST : 18243, 35400008424740.0020