Titre du document / Document title

Biocompatible in-tube solid-phase microextraction coupled to HPLC for the determination of angiotensin II receptor antagonists in human plasma and urine

Auteur(s) / Author(s)

JING NIE ^{(1 2)} ; MIN ZHANG ⁽¹⁾ ; YI FAN ⁽¹⁾ ; YI WEN ⁽¹⁾ ; BINGREN XIANG ⁽²⁾ ; FENG Yu-Qi ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Chemistry, Wuhan University, Wuhan 430072, CHINE
⁽²⁾ Center for Instrumental Analysis, China Pharmaceutical University, Nanjing 210009, CHINE

Résumé / Abstract

A poly (methacrylic acid-ethylene glycol dimethacrylate, MAA-EGDMA) monolithic capillary was used for the in-tube solid-phase microextraction (in-tube SPME) of several angiotensin II receptor antagonists (ARA-IIs) from human plasma and urine. Under the optimized extraction condition, the protein component of the biological sample was flushed through the monolithic capillary, while the analytes were successfully trapped. Coupled to HPLC with fluorescence detection, this on-line in-tube SPME method was successfully applied for the determination of candesartan, losartan, irbesartan, valsartan, telmisartan, and their detection limits were found to be 0.1-15.3 ng/mL and 0.1-15.2 ng/mL in human plasma and urine, respectively. The method was linear over the range of 0.5-200 ng/mL for telmisartan, 5-2000 ng/mL for candesartan and irbesartan, 10-2000 ng/mL for valsartan, and 50-5000 ng/mL for losartan with correlation coefficients being above 0.9985 in plasma sample and above 0.9994 in urine sample. The method reproducibility was evaluated at three concentration levels, resulting in the R.S.D. <7%. The poly (MAA-EGDMA) monolithic capillary was demonstrated to be robust and biocompatible by using direct injections of biological samples.

Revue / Journal Title

Journal of chromatography. B   ISSN 1570-0232 

Source / Source

2005, vol. 828, n^o1-2, pp. 62-69 [8 page(s) (article)] (25 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (2002) (Revue)

Mots-clés anglais / English Keywords

Aminoacid ; Non peptide compound ; Tetrazole derivatives ; AT1 angiotensin receptor ; Biphenyl derivatives ; Benzimidazole derivatives ; Renin angiotensin system ; Octapeptide ; Peptide hormone ; Antihypertensive agent ; Telmisartan ; Valsartan ; Irbesartan ; Losartan ; Candesartan ; Liquid chromatography ; Ethylene glycol ; Urine ; Blood plasma ; Biological fluid ; Human ; Angiotensin receptor ; Angiotensin II ; Angiotensin antagonist ; Quantitative analysis ; HPLC chromatography ; Solid phase microextraction ;

Mots-clés français / French Keywords

Valine dérivé ; Aminoacide ; Composé non peptide ; Tétrazole dérivé ; Récepteur angiotensine AT1 ; Biphényle dérivé ; Benzimidazole dérivé ; Système renin angiotensine ; Octapeptide ; Hormone peptide ; Antihypertenseur ; Telmisartan ; Valsartan ; Irbésartan ; Losartan ; Candésartan ; Chromatographie phase liquide ; Ethane-1,2-diol ; Urine ; Plasma sanguin ; Liquide biologique ; Homme ; Récepteur angiotensine ; Angiotensine II ; Antagoniste angiotensine ; Analyse quantitative ; Chromatographie HPLC ; Microextraction phase solide ;

Mots-clés espagnols / Spanish Keywords

Aminoácido ; Compuesto no péptido ; Tetrazol derivado ; Receptor angiotensina AT1 ; Bifenilo derivado ; Benzimidazol derivado ; Sistema renin angiotensina ; Octapéptido ; Hormona péptido ; Antihipertensivo ; Telmisartán ; Valsartán ; Irbesartán ; Losartán ; Candesartán ; Cromatografía fase líquida ; 1,2-Etanodiol ; Orina ; Plasma sanguíneo ; Líquido biológico ; Hombre ; Receptor angiotensina ; Angiotensina II ; Antagonista angiotensina ; Análisis cuantitativo ; Cromatografía HPLC ; Microextracción fase sólida ;

Mots-clés d'auteur / Author Keywords

Poly (methacrylic acid-ethylene glycol dimethacrylate) ; Monolithic capillary ; In-tube SPME ; Liquid chromatography ; Angiotensin II receptor antagonists ; Candesartan ; Losartan ; Irbesartan ; Valsartan ; Telmisartan ;

Localisation / Location

INIST-CNRS, Cote INIST : 8577 B, 35400013527073.0090