Titre du document / Document title

Type I interferons protect mice against enterovirus 71 infection

Auteur(s) / Author(s)

LIU Ming-Liang ⁽¹⁾ ; LEE Yi-Ping ⁽²⁾ ; WANG Ya-Fang ⁽²⁾ ; LEI Huan-Yao ⁽¹⁾ ; LIU Ching-Chuan ⁽³⁾ ; WANG Shih-Min ⁽³⁾ ; SU Lh-Jen ⁽⁴⁾ ; WANG Jen-Reng ⁽⁵⁾ ; YEH Trai-Ming ⁽⁵⁾ ; CHEN Shun-Hua ⁽¹⁾ ; YU Chun-Keung ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Departments of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan, 70101, TAIWAN, PROVINCE DE CHINE
⁽²⁾ Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, 70101, TAIWAN, PROVINCE DE CHINE
⁽³⁾ Department of Pediatrics, College of Medicine, National Cheng Kung University, Tainan, 70101, TAIWAN, PROVINCE DE CHINE
⁽⁴⁾ Department of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, 70101, TAIWAN, PROVINCE DE CHINE
⁽⁵⁾ Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan, 70101, TAIWAN, PROVINCE DE CHINE

Résumé / Abstract

In this study, the contribution of type I interferons (IFNs) to protection against infection with enterovirus 71 (EV71) was investigated using a murine model where the virus was administrated to neonatal Institute of Cancer Research (ICR) mice by either the intraperitoneal (i.p.) or the oral route. In i.p. inoculated mice, post-infection treatment of dexamethasone (5 mg kg^-1 at 2 or 3 days after infection) exacerbated clinical symptoms and increased the tissue viral titre. In contrast, polyriboinosinic : polyribocytidylic acid [poly(I : C); 10 or 100 μg per mouse at 12 h before infection], a potent IFN inducer, improved the survival rate and decreased the tissue viral titres after EV71 challenge, which correlated with an increase in serum IFN-a concentration, the percentage of dendritic cells, their expression of major histocompatibility complex class II molecule and IFN-a in spleen. Treatment with a neutralizing antibody for type I IFNs (10⁴ neutralizing units per mouse, 6 h before and 12 h after infection) resulted in frequent deaths and higher tissue viral load in infected mice compared with control mice. In contrast, an early administration of recombinant mouse IFN-aA (10⁴ U per mouse for 3 days starting at 0, 1 or 3 days after infection) protected the mice against EV71 infection. In vitro analysis of virus-induced death in three human cell lines showed that human type I IFNs exerted a direct protective effect on EV71. It was concluded that type I IFNs play an important role in controlling EV71 infection and replication.

Revue / Journal Title

Journal of general virology   ISSN 0022-1317   CODEN JGVIAY 

Source / Source

2005, vol. 86 (12), pp. 3263-3269 [7 page(s) (article)] (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Society for General Microbiology, Reading, ROYAUME-UNI  (1967) (Revue)

Mots-clés anglais / English Keywords

Virus ; Picornaviridae ; Vertebrata ; Mammalia ; Rodentia ; Virology ; Microbiology ; Interferon ; Enterovirus ; Mouse ;

Mots-clés français / French Keywords

Virus ; Picornaviridae ; Vertebrata ; Mammalia ; Rodentia ; Virologie ; Microbiologie ; Interféron ; Enterovirus ; Souris ;

Mots-clés espagnols / Spanish Keywords

Virus ; Picornaviridae ; Vertebrata ; Mammalia ; Rodentia ; Virología ; Microbiología ; Interferón ; Enterovirus ; Ratón ;

Localisation / Location

INIST-CNRS, Cote INIST : 13533, 35400013546172.0090